abstract

• The activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that currents passing through Kv7.2/Kv7.3 channels in cholesterol-depleted cells, by acute application of methyl-β-cyclodextrin (MβCD), were less sensitive to PIP2 dephosphorylation strategies than those of control cells, suggesting that cholesterol depletion enhances the Kv7.2/Kv7.3-PIP2 interaction. In contrast, the sensitivity of Kv7.2/Kv7.3 channels to acute membrane cholesterol depletion by MβCD was not altered in mutant channels with different apparent affinities for PIP2 © 2023 Walter de Gruyter GmbH, Berlin/Boston.

authors

• Delgado-Ramírez M.
• López-Serrano A.L.
• Meza Villanueva Ulises
• Rodríguez Menchaca Aldo Azmar
• Sánchez Armass Acuña Sergio Ramón

publication date

• 2023-01-01

funding provided via

• SEP-CONACYT [CB-284443, 975]; Universidad Autonoma de San Luis Potosi [C18-FRC-08-03.03, C19-FAI-05-59.59]  Grant

published in

• Biological Chemistry  Journal

keywords

• Cholesterol, Cyclodextrin, Ion channels, Lipids, PIP2

Digital Object Identifier (DOI)

• 10.1515/hsz-2023-0204

PubMed ID

• 37552610

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