Solvent-free enzymatic synthesis of structured lipids containing CLA from coconut oil and tricaprylin Article uri icon

abstract

  • Lipase-catalyzed acidolysis of different TAG with CLA was performed to produce structured lipids (SL) containing CLA. An immobilized lipase from Mucor miehei (Lipozyme IM, Novo Nordisk Inc., Franklinton, NC) was used as the biocatalyst in a solvent-free system. Coconut oil and tricaprylin, which are sources of medium-chain FA, were the starting substrates, and a mixture of FFA (MFFA) containing 73%25 CLA was the donor of the acyl groups. For each TAG, four different ratios of TAG/MFFA were blended to prepare about 500 g of mixture containing 10, 20, 30, and 40%25 CLA (w/w). Each blend was reacted with 5%25 lipase at 65°C for 48 h under nitrogen. Over the range of TAG/ MFFA ratios examined, CLA was incorporated effectively by the enzyme. Lipozyme IM exhibited no special preference for any particular FA, since the incorporation of FA was proportional to their concentration in the system. FFA, PV, ρ-anisidine value (p-AV), iodine value (IV), and saponification number (SN) were evaluated for all the SL. FFA, PV, and ρ-AV depended on the purification process and showed no significant deterioration of SL with respect to the original TAG, whereas IV and SN depended on the composition of the SL, mainly the CLA content.

publication date

  • 2004-01-01