Construction and Validation of a Chloroplast Expression Vector for the Production of Recombinant Proteins in Chlorella vulgaris
type: Information Content Entity
accesstype: All Open Access, Gold
artnum: 884897
freetext keyword: biopharmaceuticals; electroporation; freshwater green algae; genetic engineering; homologous recombination
type: Thing
related by: authorship_69185
abstract: Microalgae constitute a diverse group of photosynthetic unicellular microorganisms that have gained immense attention for biotechnological applications. They are promising platforms for the production of high-value metabolites and biopharmaceuticals for commercial and therapeutic applications because of their physiological properties and capability to grow easily in natural and artificial environments. Although the proof-of-concept for some applications have been achieved for model species, such as Chlamydomonas reinhardtii, the genetic engineering methods for microalgae are still in their infancy. Thus, an expansion of this field is required. Chlorella vulgaris is an important algal species with a high protein content and requires focus for the development of an efficient nuclear and chloroplast transformation process. This research aimed to establish a chloroplast transformation method for the freshwater green-algae species C. vulgaris based on a specific expression vector (pCMCC, which was named after Chula Mexico Chlorella chloroplast) constructed with endogenous recombination regions, namely, 16S–trn I (left) and trn A–23S (right), and the Prrn promoter. Human basic fibroblast growth factor (bFGF) was adopted as a target biopharmaceutical to establish the chloroplast expression approach. The plasmid pCMCC:bFGF was transformed into C. vulgaris via electroporation using simple carbohydrate-based buffers, which aided in the transfer of the transgene into the chloroplast genome. Cells transformed with the pCMCC:bFGF vector were selected using kanamycin, and resistant colonies were analyzed by polymerase chain reaction and Western blotting to confirm the presence of the transgene and the recombinant bFGF, respectively. The bFGF that accumulated in the transplastomic C. vulgaris clones ranged from 0.26 to 1.42 ng/g fresh weight of biomass, and it was quantified by enzyme-linked immunosorbent assay. Therefore, the designed expression vector, in combination with an opt…
e iscopus: 2-s2.0-85134063022
estado: published
volume: 9
type: Article
has funding vehicle: Chulalongkorn University, CU
related by: authorship_69183
type: Document
crisid: 9994
type: Entity
related by: authorship_69184
doi: 10.3389/fmars.2022.884897
type: Continuant
origen: scopus
most specific type: Document
related by: authorship_69182
has publication venue: Frontiers in Marine Science
date time value: article date 9994
most specific type: Article
type: Generically Dependent Continuant

Document
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Continuant
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Entity
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Chulalongkorn University, CU
type: Relationship
type: BFO_0000020
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type: Thing
type: Grant
type: Continuant
funding vehicle for: Construction and Validation of a Chloroplast Expression Vector for the Production of Recombinant Proteins in Chlorella vulgaris

Generically Dependent Continuant
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RDF description of Construction and Validation of a Chloroplast Expression Vector for the Production of Recombinant Proteins in Chlorella vulgaris - http://orbis.uaslp.mx/vivo/individual/scopus9993
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date: 2026-08-28T06:01:43
publisher: vivo
type: Document

Article
type: Class

Information Content Entity
type: Class

Frontiers in Marine Science
type: Thing
type: Generically Dependent Continuant
type: Collection
type: Continuant
publication venue for: Construction and Validation of a Chloroplast Expression Vector for the Production of Recombinant Proteins in Chlorella vulgaris
type: Journal
type: Document
type: Entity
type: Periodical
type: Information Content Entity