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Methodological Approach for Modeling of Multienzyme in-pot Processes
This paper presents a methodological approach for modeling multi-enzyme in-pot processes. The methodology is exemplified stepwise through the bi-enzymatic production of N-acetyl-D-neuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc). In this case study, sensitivity analysis is also used to evaluate the reliability of all parameters of the model suggested in literature [5], Results, from the sensitivity analysis, are used as criteria for a systematic simplification of the model structure. Consequently, model complexity was reduced without compromising the general predictive performance. A deviation of less than 5%25 from the original model was found. © 2011 Elsevier B.V.
Mathematical model; Multi-enzyme in-pot; Neu5Ac production
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Generically Dependent Continuant
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