Document
type: Class

Cell Calcium
type: Journal
type: Document
type: Periodical
publication venue for: Regulation of the Ca2 -activated chloride channel Anoctamin-1 (TMEM16A) by Ca2 -induced interaction with FKBP12 and calcineurin
type: Collection
type: Generically Dependent Continuant
type: Thing
type: Entity
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Regulation of the Ca2 -activated chloride channel Anoctamin-1 (TMEM16A) by Ca2 -induced interaction with FKBP12 and calcineurin
type: Entity
most specific type: Document
type: Document
type: Thing
related by: authorship_43495
crisid: 6633
related by: authorship_43496
e iscopus: 2-s2.0-85084472276
related by: authorship_43499
type: Article
chemicalscas: calcineurin, 137951-12-3; calcium ion, 14127-61-8; cyclosporine, 59865-13-3, 63798-73-2, 79217-60-0; ionomycin, 56092-81-0; rapamycin, 53123-88-9; tacrolimus, 104987-11-3
has publication venue: Cell Calcium
doi: 10.1016/j.ceca.2020.102211
estado: published
type: Generically Dependent Continuant
related by: authorship_43494
freetext keyword: Calcineurin; Calcium; Channel activation; Chloride channel; Cyclosporine A; Electrophysiology; FK506; FKBP12; Oligomerization; Rapamycin; TMEM16A anoctamin 1; calcineurin; calcium ion; cyclosporine; fk 506 binding protein; fk 506 binding protein 12; ionomycin; rapamycin; sphingosine 1 phosphate receptor; tacrolimus; unclassified drug; Article; calcium cell level; clinical effectiveness; complex formation; controlled study; drug effect; drug efficacy; drug mechanism; drug response; embryo; HEK293 cell line; human; human cell; immunoprecipitation; oligomerization; priority journal; protein expression; protein interaction
abstract: Chloride fluxes through the calcium-gated chloride channel Anoctamin-1 (TMEM16A) control blood pressure, secretion of saliva, mucin, insulin, and melatonin, gastrointestinal motility, sperm capacitation and motility, and pain sensation. Calcium activates a myriad of regulatory proteins but how these proteins affect TMEM16A activity is unresolved. Here we show by co-immunoprecipitation that increasing intracellular calcium with ionomycin or by activating sphingosine-1-phosphate receptors, induces coupling of calcium/calmodulin-dependent phosphatase calcineurin and prolyl isomerase FK506-binding protein 12 (FKBP12) to TMEM16A in HEK-293 cells. Application of drugs that target either calcineurin (cyclosporine A) or FKBP12 (tacrolimus known as FK506 and sirolimus known as rapamycin) caused a decrease in TMEM16A activity. In addition, FK506 and BAPTA-AM prevented co-immunoprecipitation between FKBP12 and TMEM16A. FK506 rendered the channel insensitive to cyclosporine A without altering its apparent calcium sensitivity whereas zero intracellular calcium blocked the effect of FK506. Rapamycin decreased TMEM16A activity in cells pre-treated with cyclosporine A or FK506. These results suggest the formation of a TMEM16A-FKBP12-calcineurin complex that regulates channel function. We conclude that upon a cytosolic calcium increase the TMEM16A-FKPB12-calcineurin trimers are assembled. Such hetero-oligomerization enhances TMEM16A channel activity but is not mandatory for activation by calcium. © 2020 Elsevier Ltd
pmid: 32422433
artnum: 102211
type: Continuant
date time value: article date 6633
label: Regulation of the Ca2%2b-activated chloride channel Anoctamin-1 (TMEM16A) by Ca2%2b-induced interaction with FKBP12 and calcineurin
type: Information Content Entity
volume: 89
related by: authorship_43500
related by: authorship_43497
most specific type: Article
origen: scopus
related by: authorship_43501
related by: authorship_43498

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RDF description of Regulation of the Ca2 -activated chloride channel Anoctamin-1 (TMEM16A) by Ca2 -induced interaction with FKBP12 and calcineurin - http://orbis.uaslp.mx/vivo/individual/scopus6632
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date: 2026-08-24T21:45:43
publisher: vivo
type: Document

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