Document
type: Class

Continuant
type: Class

Entity
type: Class

International Journal of Phytoremediation
type: Collection
type: Periodical
type: Document
type: Information Content Entity
type: Thing
type: Continuant
type: Journal
publication venue for: Increased removal of cadmium by Chlamydomonas reinhardtii modified with a synthetic gene for γ-glutamylcysteine synthetase
type: Entity
type: Generically Dependent Continuant

Generically Dependent Continuant
type: Class

RDF description of Increased removal of cadmium by Chlamydomonas reinhardtii modified with a synthetic gene for γ-glutamylcysteine synthetase - http://orbis.uaslp.mx/vivo/individual/scopus6510
rights: termsOfUse
date: 2026-08-24T21:51:14
publisher: vivo
type: Document

Article
type: Class

131777, 257616, 287506
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: Increased removal of cadmium by Chlamydomonas reinhardtii modified with a synthetic gene for γ-glutamylcysteine synthetase

Information Content Entity
type: Class

Increased removal of cadmium by Chlamydomonas reinhardtii modified with a synthetic gene for γ-glutamylcysteine synthetase
related by: authorship_42551
related by: authorship_42546
chemicalscas: cadmium, 22537-48-0, 7440-43-9; glutamate cysteine ligase, 9023-64-7; Cadmium; Glutamate-Cysteine Ligase
abstract: Bioremediation with genetically modified microalgae is becoming an alternative to remove metalloids and metals such as cadmium, a contaminant produced in industrial processes and found in domestic waste. Its removal is important in several countries including Mexico, where the San Luis Potosi region has elevated levels of it. We generated a construct with a synthetic gene for γ-glutamylcysteine synthetase and employed it in the chloroplast transformation of Chlamydomonas reinhardtii. In dose-response kinetics with media containing from 1 to 20 mg/L of cadmium, both the transplastomic clone and the wild-type strain grew similarly, but the former removed up to 32%25 more cadmium. While the growth of both decreased with higher concentrations of cadmium, the transplastomic clone removed 20 ± 9%25 more than the wild-type strain. Compared to the wild-type strain, in the transplastomic clone the activity of glutathione S-transferase and the intracellular glutathione increased up to 2.1 and 1.9 times, respectively, in media with 2.5 and 10 mg/mL of cadmium. While 20 mg/L of cadmium inhibited the growth of both, the transplastomic clone gradually duplicated. These results confirm the expression of the synthetic gene gshA in the transformed strain as revealed in its increased removal uptake and metabolic response. © 2020 Taylor %26 Francis Group, LLC.
estado: published
related by: authorship_42547
type: Entity
volume: 22
origen: scopus
issue: 12
related by: authorship_42552
type: Document
date time value: article date 6511
most specific type: Document
related by: authorship_42553
pmid: 32449363
type: Generically Dependent Continuant
related by: authorship_42549
type: Thing
freetext keyword: Bioremediation; glutathione; microalgae; transferase; transplastomic cadmium; glutamate cysteine ligase; synthetic DNA; bioremediation; Chlamydomonas reinhardtii; genetics; Mexico; Biodegradation, Environmental; Cadmium; Chlamydomonas reinhardtii; Genes, Synthetic; Glutamate-Cysteine Ligase; Mexico
has publication venue: International Journal of Phytoremediation
page start: 1269
type: Article
type: Information Content Entity
crisid: 6511
e iscopus: 2-s2.0-85086004338
doi: 10.1080/15226514.2020.1765138
abstract: Bioremediation with genetically modified microalgae is becoming an alternative to remove metalloids and metals such as cadmium, a contaminant produced in industrial processes and found in domestic waste. Its removal is important in several countries including Mexico, where the San Luis Potosi region has elevated levels of it. We generated a construct with a synthetic gene for γ-glutamylcysteine synthetase and employed it in the chloroplast transformation of Chlamydomonas reinhardtii. In dose-response kinetics with media containing from 1 to 20 mg/L of cadmium, both the transplastomic clone and the wild-type strain grew similarly, but the former removed up to 32%25 more cadmium. While the growth of both decreased with higher concentrations of cadmium, the transplastomic clone removed 20 ± 9%25 more than the wild-type strain. Compared to the wild-type strain, in the transplastomic clone the activity of glutathione S-transferase and the intracellular glutathione increased up to 2.1 and 1.9 times, respectively, in media with 2.5 and 10 mg/mL of cadmium. While 20 mg/L of cadmium inhibited the growth of both, the transplastomic clone gradually duplicated. These results confirm the expression of the synthetic gene gshA in the transformed strain as revealed in its increased removal uptake and metabolic response. © 2020 Taylor & Francis Group, LLC.
related by: authorship_42548
type: Continuant
has funding vehicle: 131777, 257616, 287506
related by: authorship_42550
page end: 1277
most specific type: Article