Consejo Nacional de Ciencia y Tecnología, CONCYT
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Consejo Nacional de Ciencia y Tecnología, CONACYT: CVU 372299
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RDF description of Low levels of alpha-synuclein in peripheral tissues are related to clinical relapse in relapsing-remitting multiple sclerosis: a pilot cross-sectional study - http://orbis.uaslp.mx/vivo/individual/scopus6199
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Flow cytometry; Immunohistochemistry; Multiple sclerosis; Peripheral blood mononuclear cells; Skin; α-Synuclein alpha synuclein; methylprednisolone; alpha synuclein; CD207 protein, human; lectin; leukocyte antigen; mannose binding lectin; steroid; adult; Article; blood sampling; clinical article; controlled study; cross-sectional study; epidermis; Expanded Disability Status Scale; female; flow cytometry; human; immunohistochemistry; Langerhans cell; lymphocyte; male; monocyte; multiple sclerosis; peripheral blood mononuclear cell; pilot study; priority journal; protein localization; relapse; skin; skin biopsy; biopsy; blood; blood cell; drug effect; follow up; Langhans giant cell; metabolism; middle aged; multiple sclerosis; nonparametric test; nucleolus; pathology; skin; ultrastructure; young adult; Adult; alpha-Synuclein; Antigens, CD; Biopsy; Blood Cells; Cell Nucleolus; Cross-Sectional Studies; Female; Flow Cytometry; Follow-Up Studies; Giant Cells, Langhans; Humans; Lectins, C-Type; Male; Mannose-Binding Lectins; Middle Aged; Multiple Sclerosis, Relapsing-Remitting; Pilot Projects; Skin; Statistics, Nonparametric; Steroids; Young Adult
Low levels of alpha-synuclein in peripheral tissues are related to clinical relapse in relapsing-remitting multiple sclerosis: a pilot cross-sectional study
The protein alpha-synuclein (α-Syn) has been linked to neuroinflammatory conditions. We investigated whether the presence of α-Syn in peripheral tissues is a surrogate of brain inflammatory status in a small group of relapsing-remitting multiple sclerosis (RRMS) patients in a pilot cross-sectional study. Skin biopsies and peripheral blood were sampled from 34 healthy controls and 23 MS patients for measurement of α-Syn levels. Within the RRMS group 15 patients were in remission, and 8 patients were in the relapsing phase. The protein α-Syn was evaluated by means of immunohistochemistry and flow cytometry in skin and nucleated blood cells, respectively. In the skin, α-Syn levels were lower in relapsing MS than in the other groups, both in positive area (p =.021) and staining intensity (p =.004). In blood, the percentage of α-Syn-positive lymphocytes and monocytes were not statistically different between study groups. Moreover, the use of systemic steroids did not affect α-Syn positivity in MS-relapse patients. Finally, epidermic Langerhans cells did not stain positively for α-Syn. Overall, the levels of α-Syn positivity were lower in inflammatory relapse of RRMS patients when measured in peripheral tissues. We discuss the role of α-Syn levels in inflammation according to the obtained results. © 2018 Elsevier B.V.
alpha synuclein, 154040-18-3; methylprednisolone, 6923-42-8, 83-43-2; alpha-Synuclein; Antigens, CD; CD207 protein, human; Lectins, C-Type; Mannose-Binding Lectins; Steroids
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