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RDF description of Increased levels of adipose tissue-resident Th17 cells in obesity associated with miR-326 - http://orbis.uaslp.mx/vivo/individual/scopus6179
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bisulfite, 15181-46-1, 7631-90-5, 7773-03-7; gamma interferon, 82115-62-6; interleukin 2, 85898-30-2; Forkhead Transcription Factors; FOXP3 protein, human; MicroRNAs; MIRN155 microRNA, human; MIRN326 microRNA, human; Nuclear Receptor Subfamily 1, Group F, Member 3; RORC protein, human
miRNAs are important immune regulators in the control of the CD4 T cells phenotype. miR-326 regulates the differentiation towards Th17 cells and the inhibition of miR-155 is associated with low levels of Treg cells. However, miRNAs expression and transcription factors associated with these lymphocyte subsets in obesity-induced adipose tissue inflammation is still unknown. The aim of this work was to identify Th17 cells in subcutaneous adipose tissue (SAT), proinflammatory cytokine production and their association with the miRNAs and transcription factors involved. We collected SAT samples obtained by lipoaspiration from individuals with normal weight, overweight and obesity. We obtained the stromal vascular fractions and then a Ficoll gradient was performed to obtain adipose tissue mononuclear cells (ATMC). Th17 cells were evaluated by flow cytometry and the expression of miR-326, miR-155, RORC2 and FOXP3 by qRT-PCR. We also analyzed cytokines from the supernatants of the ATMC culture and measured the FOXP3 methylation percentage by bisulfite conversion by PCR. According to the results, the frequency of Th17 cells and RORC2 expression was higher in individuals with obesity and associated with miR-326 expression. The ATMC from this group secreted a proinflammatory cytokine profile by in vitro assay. In contrast, lower levels of mRNA FOXP3 expression was detected in ATMC from individuals with obesity that correlated with methylation percentage of FOXP3 gene but no association with miR-155 was detected. Our results suggested that miR-326 participates in the polarization towards Th17 promoting the inflammatory state in the obesity-induced adipose tissue. © 2019 European Federation of Immunological Societies
Increased levels of adipose tissue-resident Th17 cells in obesity associated with miR-326
Adipose tissue; FOXP3 methylation; Inflammation; miR-155; miR-326; Obesity; Th17 cells; Transcription factors biological marker; bisulfite; gamma interferon; interleukin 10; interleukin 17; interleukin 1beta; interleukin 2; interleukin 6; messenger RNA; microRNA; microRNA 155; microRNA 326; retinoid related orphan receptor gamma; retinoid related orphan receptor gamma 2; transcription factor FOXP3; tumor necrosis factor; unclassified drug; forkhead transcription factor; FOXP3 protein, human; microRNA; MIRN155 microRNA, human; MIRN326 microRNA, human; retinoid related orphan receptor gamma; RORC protein, human; adipose tissue mononuclear cell; adult; Article; body mass; CD4 lymphocyte count; cell function; cell polarity; cellular distribution; clinical article; controlled study; cytokine production; cytokine release; disease marker; female; flow cytometry; FOXP3 gene; gene; gene control; gene expression; gene function; gene location; gene sequence; genetic association; genetic transcription; human; human tissue; immunocompetent cell; immunoregulation; in vitro study; inflammation; intron; male; mononuclear cell; obesity; pathophysiology; peripheral blood mononuclear cell; priority journal; protein expression; protein function; protein localization; protein methylation; quantitative analysis; regulatory T lymphocyte; reliability; reverse transcription polymerase chain reaction; signal transduction; subcutaneous fat; Th17 cell; tissue distribution; adipose tissue; cell culture; cell differentiation; gene expression regulation; genetics; immunology; lymphocyte activation; metabolism; middle aged; obesity; Th17 cell; young adult; Adipose Tissue; Adult; Cell Differentiation; Cells, Cultured; Female; Forkhead Transcription Factors; Gene Expression Regulation; Humans; Lymphocyte Activation; Male; MicroRNAs; Middle Aged; Nuclear Receptor Subfamily 1, Group F, Member 3; Obesity; T-Lymphocytes, Regulatory; Th17 Cells; Young Adult
miRNAs are important immune regulators in the control of the CD4 %2b T cells phenotype. miR-326 regulates the differentiation towards Th17 cells and the inhibition of miR-155 is associated with low levels of Treg cells. However, miRNAs expression and transcription factors associated with these lymphocyte subsets in obesity-induced adipose tissue inflammation is still unknown. The aim of this work was to identify Th17 cells in subcutaneous adipose tissue (SAT), proinflammatory cytokine production and their association with the miRNAs and transcription factors involved. We collected SAT samples obtained by lipoaspiration from individuals with normal weight, overweight and obesity. We obtained the stromal vascular fractions and then a Ficoll gradient was performed to obtain adipose tissue mononuclear cells (ATMC). Th17 cells were evaluated by flow cytometry and the expression of miR-326, miR-155, RORC2 and FOXP3 by qRT-PCR. We also analyzed cytokines from the supernatants of the ATMC culture and measured the FOXP3 methylation percentage by bisulfite conversion by PCR. According to the results, the frequency of Th17 cells and RORC2 expression was higher in individuals with obesity and associated with miR-326 expression. The ATMC from this group secreted a proinflammatory cytokine profile by in vitro assay. In contrast, lower levels of mRNA FOXP3 expression was detected in ATMC from individuals with obesity that correlated with methylation percentage of FOXP3 gene but no association with miR-155 was detected. Our results suggested that miR-326 participates in the polarization towards Th17 promoting the inflammatory state in the obesity-induced adipose tissue. © 2019 European Federation of Immunological Societies