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Consejo Nacional de Ciencia y Tecnología, CONACYT: 695756
Generically Dependent Continuant
Plasmonic photothermal therapy (PPTT) has been used as an alternative to chemotherapy for the elimination of resistant microorganisms; however, its in situ evaluation has not been well studied. In the present study, we assessed the antimicrobial activity of a chitosan-based hydrogel embedded with gold nanorods (Ch/AuNRs) using a low power infrared diode laser. The antibacterial activity was measured in both Gram-positive and –negative strains, including clinical isolates of multidrug-resistant pathogens. The cytotoxic effect, cellular proliferation, and the expression of the pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines were quantified in a murine model of macrophages. Results showed a potent antimicrobial activity of the Ch/AuNRs with MICs ≤4 μg/mL, very low cytotoxicity with cell viability above 80%25, and the macrophage proliferation was not affected for a period of 48 h. These results suggest that our Ch/AuNR-embedded hydrogel could be an option to locally control chronic nosocomial infections using PPTT. © 2019 Elsevier Inc.
Antibacterial; Antifungal; Chitosan; Cytotoxicity; Gold nanorod; Inflammation; Multidrug resistant; Photothermal therapy Cell proliferation; Chemotherapy; Chitosan; Cytotoxicity; Gold; Hydrogels; Macrophages; Nanorods; Petroleum reservoir evaluation; Plasmonics; Anti-fungal; Antibacterial; Gold nanorod; Inflammation; Multidrug resistants; Photothermal therapy; Microorganisms; amikacin; ceftazidime; ceftizoxime; ciprofloxacin; clindamycin; ertapenem; erythromycin; gold nanorod; interleukin 10; interleukin 6; oxacillin; penicillin derivative; piperacillin plus tazobactam; tobramycin; tumor necrosis factor; antifungal agent; antiinfective agent; broxuridine; chitosan; gold; nanotube; Acinetobacter baumannii; antifungal activity; antimicrobial activity; Article; bacterial strain; broth dilution; Candida albicans; cell proliferation; cell viability; controlled study; Cryptococcus neoformans; disk diffusion; hydrogel; immune response; Klebsiella pneumoniae; methicillin resistant Staphylococcus aureus; minimum bactericidal concentration; minimum fungicidal concentration; nonhuman; photothermal therapy; plasmonic photothermal therapy; protein expression; Pseudomonas aeruginosa; RAW 264.7 cell line; scanning electron microscopy; Staphylococcus aureus; Staphylococcus epidermidis; transmission electron microscopy; Trichophyton mentagrophytes; ultraviolet spectrophotometry; X ray spectroscopy; animal; cell death; chemistry; drug effect; hydrogel; inflammation; metabolism; microbial sensitivity test; mouse; pathology; pharmacology; phototherapy; thermotherapy; ultrastructure; Animals; Anti-Infective Agents; Antifungal Agents; Bromodeoxyuridine; Cell Death; Cell Proliferation; Chitosan; Gold; Hydrogels; Hyperthermia, Induced; Inflammation; Mice; Microbial Sensitivity Tests; Nanotubes; Phototherapy; RAW 264.7 Cells
Hydrogel-embedded gold nanorods activated by plasmonic photothermy with potent antimicrobial activity
Nanomedicine: Nanotechnology, Biology, and Medicine
RDF description of Hydrogel-embedded gold nanorods activated by plasmonic photothermy with potent antimicrobial activity - http://orbis.uaslp.mx/vivo/individual/scopus6170
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