Exposure of NIH/3T3 fibroblasts not expressing P-glycoprotein to 50, 30, 20, and 10%25 hyposmotic solutions led to cell volume increases of 70, 32, 21, and 12%25, respectively. After swelling, NIH/3T3 cells exhibited regulatory volume decrease (RVD), attaining complete volume recovery after 30 min except in 50%25 hyposmotic solution, in which volume recovery was 76%25. RVD was accelerated by gramicidin and inhibited by the Cl channel blockers 5-nitro-2(3-phenylpropylamino)-benzoic acid, 1,9-dideoxyforskolin, dipyridamole, and niflumic acid and by the K channel blocker quinidine. RVD was reduced 15%25 by removal of extracellular Ca. The pathway opened by hypotonicity was highly permeable to K and Rb and only partly permeable to other cations. Most anions were able to permeate, with a permeability ranking of nitrate > benzoate = iodide > thiocyanate > chloride >> gluconate. The pathway was permeable to neutral amino acids, with a permeability ranking of glycine > alanine > glutamate > taurine > γ- aminobutyric acid > glutamine. The pathway was not permeable to basic amino acids. These results show that, despite the absence of P-glycoprotein, NIH/3T3 cells exhibit RVD with properties similar to those expressed in most cell types.
Amino acids; Chloride channels; Hyposmolarity anion; chloride channel; glycoprotein P; animal cell; anion transport; article; cell membrane permeability; cell strain 3T3; cell volume; controlled study; mouse; nonhuman; osmolarity; priority journal; Animalia
Volume regulation in NIH/3T3 cells not expressing P-glycoprotein. I. Regulatory volume decrease
Document
American Journal of Physiology - Cell Physiology
Generically Dependent Continuant
RDF description of Volume regulation in NIH/3T3 cells not expressing P-glycoprotein. I. Regulatory volume decrease - http://orbis.uaslp.mx/vivo/individual/scopus612
Article
Information Content Entity