Document
type: Class

Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper
doi: 10.1016/j.fsi.2016.07.014
estado: published
origen: scopus
has funding vehicle: CB-2010/155381, CONACYT-INFR-2014-01/225924, CONACYT-PDCPN2014-01/248033
related by: authorship_28646
has publication venue: Fish and Shellfish Immunology
e iscopus: 2-s2.0-84979690581
related by: authorship_28643
volume: 56
type: Entity
type: Article
type: Thing
related by: authorship_28645
pmid: 27417232
type: Document
type: Generically Dependent Continuant
chemicalscas: Bacterial Toxins; Fish Proteins
type: Continuant
crisid: 4799
related by: authorship_28647
most specific type: Document
page end: 321
freetext keyword: Gene expression; Humoral immune parameters; Lysate Vibrio parahaemolyticus; Pacific red snapper; ToxA; Vibrio parahaemolyticus bacterial toxin; fish protein; animal; antibody specificity; fish disease; gene expression; genetics; humoral immunity; immunology; microbiology; mucus; Perciformes; physiology; serum; veterinary; Vibrio parahaemolyticus; vibriosis; Animals; Bacterial Toxins; Fish Diseases; Fish Proteins; Gene Expression; Immunity, Humoral; Mucus; Organ Specificity; Perciformes; Serum; Vibrio Infections; Vibrio parahaemolyticus
related by: authorship_28642
date time value: article date 4799
abstract: Immunogenicity of ToxA and Vibrio parahaemolyticus lysate was evaluated in a double immunostimulation scheme in Pacific red snapper after V. parahaemolyticus infection. Three groups of Pacific red snapper were intraperitonealy (i.p.) injected with phosphate-buffered saline (PBS group), ToxA of V. parahaemolyticus (ToxA-Vp group) or V. parahaemolyticus lysate (lysate-Vp group) (first injection, day 1; second injection, day 7). Fish were subsequently infected with live V. parahaemolyticus. Humoral immune parameters in skin mucus and serum were evaluated on days 1, 7, 8 and 14 days post-immunostimulation and 7 days post-infection. Moreover expression of immune-related genes was quantified by real time PCR in head-kidney leukocytes, spleen, liver, and intestine. The ToxA-Vp-treated group showed a higher anti-protease and catalase activity in skin mucus when compared with the PBS group. Measurements of SOD and CAT activities showed an increment in both activities a day after the second boost with ToxA-Vp or lysate-Vp. Interestingly, IgM levels in mucus and transcripts were enhanced followed the ToxA-Vp treatment even after challenge. Furthermore, IL-1β was strongly expressed in all analyzed cell or tissues followed ToxA-Vp or Vp-lysate treatments. Finally, SOD and CAT gene expression was up-regulated in fish immunostimulated with either treatment ToxA-Vp or lysate-Vp, mainly after infection in head-kidney leukocytes and intestine. This is the first study where the effects of ToxA from V. parahaemolyticus in the immune system of Pacific red snapper was evaluated. These results suggest that ToxA-Vp would positively affect humoral immune response and up-regulate expression of genes involved in the immune system function; and could help in the control of V. parahaemolyticus infection in Pacific red snapper Lutjanus peru, an economic important fish in Mexico. © 2016 Elsevier Ltd
related by: authorship_28648
most specific type: Article
related by: authorship_28644
page start: 310
related by: authorship_28641
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CB-2010/155381, CONACYT-INFR-2014-01/225924, CONACYT-PDCPN2014-01/248033
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funding vehicle for: Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper

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RDF description of Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper - http://orbis.uaslp.mx/vivo/individual/scopus4798
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date: 2026-08-27T06:20:39
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Fish and Shellfish Immunology
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publication venue for: Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper
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