Circulating microvesicles regulate treg and Th17 differentiation in human autoimmune thyroid disorders
abstract: Background: Microvesicles (MVs) are emerging as important contributors to the development of inflammatory and autoimmune diseases. MVs can mediate immune modulation carrying genetic information, including microRNAs that can be transferred between cells. Design:Wedetermined the plasma levels of annexin-V MVs derived from different immune cells and platelets in patients with autoimmune thyroid diseases (AITDs) and in healthy controls. T lymphocyte polarization assays were performed in the presence of MVs to evaluate their effect in T regulatory and T helper 17 cells differentiation. microRNA content into plasma MVs and their corresponding mRNA targets were evaluated by RT-PCR. Results: The percentage of platelet-derived MVs (CD41a ) was significantly increased in plasma samples from AITD patients compared with healthy controls. In contrast, patients with AITD showeda lower percentage of leukocyteandendothelial cell-derivedMVscompared with controls. In addition, functional assays showed that MVs from AITD patients inhibited the in vitro differentiation of Foxp3 T regulatory cells (11.35%25 vs 4.40%25, P = .01) and induced the expression of interferon-γ by CD4 lymphocytes (10.91%25 vs 13.99%25, P = .01) as well as the differentiation of T helper 17 pathogenic (IL-17 interferon-γ ) cells (1.98%25 vs 5.13%25, P = .03). Furthermore, in AITD patients, whereas miR-146a and miR-155 were increased in circulating MVs, their targets IL-8 and SMAD4 were decreased in peripheral blood mononuclear cells. Conclusions: Our data indicate that circulating MVs seem to have a relevant role in the modulation of the inflammatory response observed in AITD. Copyright © 2015 by the Endocrine Society.
accesstype: All Open Access, Bronze
crisid: 4260
abstract: Background: Microvesicles (MVs) are emerging as important contributors to the development of inflammatory and autoimmune diseases. MVs can mediate immune modulation carrying genetic information, including microRNAs that can be transferred between cells. Design:Wedetermined the plasma levels of annexin-V%2bMVs derived from different immune cells and platelets in patients with autoimmune thyroid diseases (AITDs) and in healthy controls. T lymphocyte polarization assays were performed in the presence of MVs to evaluate their effect in T regulatory and T helper 17 cells differentiation. microRNA content into plasma MVs and their corresponding mRNA targets were evaluated by RT-PCR. Results: The percentage of platelet-derived MVs (CD41a%2b) was significantly increased in plasma samples from AITD patients compared with healthy controls. In contrast, patients with AITD showeda lower percentage of leukocyteandendothelial cell-derivedMVscompared with controls. In addition, functional assays showed that MVs from AITD patients inhibited the in vitro differentiation of Foxp3%2b T regulatory cells (11.35%25 vs 4.40%25, P = .01) and induced the expression of interferon-γ by CD4%2b lymphocytes (10.91%25 vs 13.99%25, P = .01) as well as the differentiation of T helper 17 pathogenic (IL-17%2binterferon-γ%2b) cells (1.98%25 vs 5.13%25, P = .03). Furthermore, in AITD patients, whereas miR-146a and miR-155 were increased in circulating MVs, their targets IL-8 and SMAD4 were decreased in peripheral blood mononuclear cells. Conclusions: Our data indicate that circulating MVs seem to have a relevant role in the modulation of the inflammatory response observed in AITD. Copyright © 2015 by the Endocrine Society.
page start: E1531
freetext keyword: gamma interferon; interleukin 8; lipocortin 5; messenger RNA; microRNA; microRNA 146a; microRNA 155; Smad4 protein; IL8 protein, human; interleukin 8; messenger RNA; microRNA; MIRN146 microRNA, human; MIRN155 microRNA, human; Smad4 protein; SMAD4 protein, human; apoptosis; Article; CD4 T lymphocyte; controlled study; endothelium cell; flow cytometry; Graves disease; Hashimoto disease; human; human cell; immunoblotting; immunocompetent cell; in vitro study; leukocyte; lymphocyte differentiation; major clinical study; membrane microparticle; peripheral blood mononuclear cell; priority journal; protein blood level; protein expression; regulatory T lymphocyte; reverse transcription polymerase chain reaction; Th17 cell; thrombocyte; autoimmune thyroiditis; blood; cell differentiation; chemistry; cytoplasm vesicle; metabolism; pathology; polymerase chain reaction; regulatory T lymphocyte; Th17 cell; Apoptosis; Blood Platelets; CD4-Positive T-Lymphocytes; Cell Differentiation; Cytoplasmic Vesicles; Flow Cytometry; Humans; Interleukin-8; MicroRNAs; Polymerase Chain Reaction; RNA, Messenger; Smad4 Protein; T-Lymphocytes, Regulatory; Th17 Cells; Thyroiditis, Autoimmune
related by: authorship_25020
related by: authorship_25024
type: Information Content Entity
type: Entity
pmid: 26480286
type: Thing
related by: authorship_25025
freetext keyword: gamma interferon; interleukin 8; lipocortin 5; messenger RNA; microRNA; microRNA 146a; microRNA 155; Smad4 protein; IL8 protein, human; interleukin 8; messenger RNA; microRNA; MIRN146 microRNA, human; MIRN155 microRNA, human; Smad4 protein; SMAD4 protein, human; apoptosis; Article; CD4+ T lymphocyte; controlled study; endothelium cell; flow cytometry; Graves disease; Hashimoto disease; human; human cell; immunoblotting; immunocompetent cell; in vitro study; leukocyte; lymphocyte differentiation; major clinical study; membrane microparticle; peripheral blood mononuclear cell; priority journal; protein blood level; protein expression; regulatory T lymphocyte; reverse transcription polymerase chain reaction; Th17 cell; thrombocyte; autoimmune thyroiditis; blood; cell differentiation; chemistry; cytoplasm vesicle; metabolism; pathology; polymerase chain reaction; regulatory T lymphocyte; Th17 cell; Apoptosis; Blood Platelets; CD4-Positive T-Lymphocytes; Cell Differentiation; Cytoplasmic Vesicles; Flow Cytometry; Humans; Interleukin-8; MicroRNAs; Polymerase Chain Reaction; RNA, Messenger; Smad4 Protein; T-Lymphocytes, Regulatory; Th17 Cells; Thyroiditis, Autoimmune
type: Document
origen: scopus
has publication venue: Journal of Clinical Endocrinology and Metabolism
type: Continuant
estado: published
chemicalscas: gamma interferon, 82115-62-6; interleukin 8, 114308-91-7; lipocortin 5, 111237-10-6; Smad4 protein, 282562-18-9; IL8 protein, human; Interleukin-8; MicroRNAs; MIRN146 microRNA, human; MIRN155 microRNA, human; RNA, Messenger; Smad4 Protein; SMAD4 protein, human
e iscopus: 2-s2.0-84951001404
related by: authorship_25023
related by: authorship_25022
volume: 100
date time value: article date 4260
most specific type: Document
most specific type: Article
related by: authorship_25019
type: Article
page end: E1539
doi: 10.1210/jc.2015-3146
related by: authorship_25021
type: Generically Dependent Continuant
issue: 12
related by: authorship_25026

Document
type: Class

Continuant
type: Class

Entity
type: Class

Generically Dependent Continuant
type: Class

RDF description of Circulating microvesicles regulate treg and Th17 differentiation in human autoimmune thyroid disorders - http://orbis.uaslp.mx/vivo/individual/scopus4259
rights: termsOfUse
date: 2026-08-24T21:45:39
publisher: vivo
type: Document

Article
type: Class

Journal of Clinical Endocrinology and Metabolism
type: Entity
type: Continuant
type: Generically Dependent Continuant
type: Journal
type: Information Content Entity
type: Periodical
type: Document
type: Thing
type: Collection
publication venue for: Circulating microvesicles regulate treg and Th17 differentiation in human autoimmune thyroid disorders

Information Content Entity
type: Class