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Consejo Nacional de Ciencia y Tecnología, CONACYT: 102109, 151480
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RDF description of Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii - http://orbis.uaslp.mx/vivo/individual/scopus3980
Article
Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii
Biofuel; Chlamydomonas reinhardtii; Lipid; Transcription factor Biochemistry; Biofuels; Biosynthesis; Genes; Lipids; Seed; Strain; Transcription; Transcription factors; Biochemistry; Biofuels; Biosynthesis; Gene expression; Genes; Lipids; Plants (botany); Seed; Transcription; Transcription factors; Biofuel synthesis; Chlamydomonas reinhardtii; Differential expressions; Fatty acid biosynthesis; Fatty acid profiles; Genetic modifications; Lipid accumulations; Transcription profiles; Genetically modified; Fatty acids; Fatty acids; fatty acid; glycerolipid; synthetic DNA; transcription factor; transcription factor Dof; unclassified drug; biofuel; lipid; transcription factor; lipid; algal genetics; algal growth; article; Chlamydomonas reinhardtii; chloroplast; controlled study; DNA modification; fatty acid analysis; fatty acid synthesis; gene overexpression; lipid analysis; lipid storage; lipogenesis; nonhuman; nucleotide sequence; phylogeny; plasmid; polymerase chain reaction; priority journal; quantitative analysis; real time polymerase chain reaction; transgenic organism; biosynthesis; Chlamydomonas reinhardtii; gene expression regulation; genetics; lipid metabolism; metabolism; photosynthesis; plant seed; amino acid sequence; Article; gas chromatography; gene overexpression; genetic transcription; monophyly; Biofuels; Chlamydomonas reinhardtii; Chloroplasts; Gene Expression Regulation, Plant; Lipid Metabolism; Lipids; Photosynthesis; Seeds; Transcription Factors; algae; Chlamydomonas reinhardtii
The high demand for less polluting, newer, and cheaper fuel resources has increased the search of the most innovative options for the production of the so-called biofuels. Chlamydomonas reinhardtii is a photosynthetic unicellular algae with multiple biotechnological advantages such as easy handling in the laboratory, a simple scale-up to industrial levels, as well as a feasible genetic modification at nuclear and chloroplast levels. Besides, its fatty acids can be used to produce biofuels. Previous studies in plants have found that the over expression of DOF-type transcription factor genes increases the synthesis and the accumulation of total lipids in seeds. In this context, the over-expression of a DOF-type transcription factor in C. reinhardtii was applied as approach to increase the amount of lipids. The results indicate higher amounts (around 2-fold) of total lipids, which are mainly fatty acids, in the genetically C. reinhardtii modified strains when compared with the non-genetically modified strain. In order to elucidate the possible function of the introduced Dof-type transcription factor, we performed a transcription profile of 8 genes involved in fatty acid biosynthesis and 6 genes involved in glycerolipid biosynthesis, by quantitative real time (qRT-PCR). Differential expression profile was observed, which can explain the increase in lipid accumulation. However, these strains did not show notable changes in the fatty acid profile. This work represents an early effort in generating a strategy to increase fatty acids production in C. reinhardtii and their use in biofuel synthesis. © 2014 Elsevier B.V.
lipid, 66455-18-3; Biofuels; Lipids; Transcription Factors
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