Document
Generically Dependent Continuant
RDF description of Chemotherapy pro-drug activation by biocatalytic virus-like nanoparticles containing cytochrome P450 - http://orbis.uaslp.mx/vivo/individual/scopus3970
Article
This work shows, for the first time, the encapsulation of a highly relevant protein in the biomedical field into virus-like particles (VLPs). A bacterial CYP variant was effectively encapsulated in VLPs constituted of coat protein from cowpea chlorotic mottle virus (CCMV). The catalytic VLPs are able to transform the chemotherapeutic pro-drug, tamoxifen, and the emerging pro-drug resveratrol. The chemical nature of the products was identified, confirming similar active products than those obtained with human CYP. The enzymatic VLPs remain stable after the catalytic reaction. The potential use of these biocatalytic nanoparticles as targeted CYP carriers for the activation of chemotherapy drugs is discussed. © 2014 Elsevier Inc.
Bionanotechnology; Catalytic VLPs; CYP; Resveratrol; Tamoxifen Catalysis; Catalyst activity; Chemotherapy; Nanoparticles; Proteins; Resveratrol; Biomedical fields; Bionanotechnology; Catalytic reactions; Catalytic VLPs; Cowpea chlorotic mottle virus; CYP; Tamoxifen; Virus-like particles; Viruses; 3,4' dihydroxytamoxifen; afimoxifene; antineoplastic agent; coat protein; cyclophosphamide; cytochrome P450; dacarbazine; endoxifen; hydrogen peroxide; ifosfamide; isopropyl thiogalactoside; n demethylase; nanocarrier; nortamoxifen; pentoxyresorufin; prodrug; resveratrol; syringol; tamoxifen; tegafur; unclassified drug; antineoplastic agent; bacterial protein; capsid protein; cytochrome P450; drug carrier; nanocapsule; nanoparticle; prodrug; resveratrol; stilbene derivative; tamoxifen; article; Bacillus megaterium; biocatalysis; cancer chemotherapy; cowpea chlorotic mottle virus; dealkylation; drug activation; drug delivery system; drug metabolism; drug transformation; encapsulation; enzyme activity; Escherichia coli; nonhuman; plant virus; protein expression; transmission electron microscopy; virus like agent; biocatalysis; Bromovirus; chemistry; drug delivery system; genetics; human; kinetics; metabolic activation; metabolism; ultrastructure; virion; Activation, Metabolic; Antineoplastic Agents; Bacillus megaterium; Bacterial Proteins; Biocatalysis; Bromovirus; Capsid Proteins; Cytochrome P-450 Enzyme System; Drug Carriers; Drug Delivery Systems; Humans; Kinetics; Nanocapsules; Nanoparticles; Prodrugs; Stilbenes; Tamoxifen; Virion
Chemotherapy pro-drug activation by biocatalytic virus-like nanoparticles containing cytochrome P450
afimoxifene, 65213-48-1, 68392-35-8; cyclophosphamide, 50-18-0; cytochrome P450, 9035-51-2; dacarbazine, 4342-03-4; endoxifen, 110025-28-0, 112093-28-4; hydrogen peroxide, 7722-84-1; ifosfamide, 3778-73-2; isopropyl thiogalactoside, 26112-89-0; nortamoxifen, 31750-48-8; pentoxyresorufin, 87687-03-4; resveratrol, 501-36-0; syringol, 91-10-1; tamoxifen, 10540-29-1; tegafur, 17902-23-7; Antineoplastic Agents; Bacterial Proteins; Capsid Proteins; Cytochrome P-450 Enzyme System; Drug Carriers; Nanocapsules; Prodrugs; resveratrol; Stilbenes; Tamoxifen
Information Content Entity