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The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions
related by: authorship_22077
type: Generically Dependent Continuant
has funding vehicle: National Center for Research Resources, NCRR: S10RR023057
related by: authorship_22078
type: Continuant
type: Article
volume: 426
date time value: article date 3826
type: Thing
freetext keyword: cowpea chlorotic mottle virus; packaging; self-assembly; ssRNA; virus-like particle capsid protein; single stranded RNA; virus RNA; article; cowpea; cowpea chlorotic mottle virus; electron microscopy; ionic strength; nonhuman; pH; priority journal; protein assembly; protein protein interaction; protein RNA binding; sedimentation rate; sucrose density gradient centrifugation; virus capsid; virus genome; virus like agent; virus nucleocapsid; Article; controlled study; cowpea chlorotic mottle virus; in vitro study; ionic strength; RNA virus; virus assembly; Cowpea chlorotic mottle virus; Miridae; RNA viruses; cowpea chlorotic mottle virus; packaging; self-assembly; ssRNA; virus-like particle; Bromovirus; Capsid Proteins; Microscopy, Electron; RNA, Viral; Virus Assembly
related by: authorship_22079
pmid: 24148696
crisid: 3826
has funding vehicle: CHE 1051507
type: Entity
has publication venue: Journal of Molecular Biology
origen: scopus
most specific type: Document
e iscopus: 2-s2.0-84894026358
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type: Information Content Entity
most specific type: Article
accesstype: All Open Access, Green
abstract: The strength of attraction between capsid proteins (CPs) of cowpea chlorotic mottle virus (CCMV) is controlled by the solution pH. Additionally, the strength of attraction between CP and the single-stranded RNA viral genome is controlled by ionic strength. By exploiting these properties, we are able to control and monitor the in vitro co-assembly of CCMV CP and single-stranded RNA as a function of the strength of CP-CP and CP-RNA attractions. Using the techniques of velocity sedimentation and electron microscopy, we find that the successful assembly of nuclease-resistant virus-like particles (VLPs) depends delicately on the strength of CP-CP attraction relative to CP-RNA attraction. If the attractions are too weak, the capsid cannot form; if they are too strong, the assembly suffers from kinetic traps. Separating the process into two steps - by first turning on CP-RNA attraction and then turning on CP-CP attraction - allows for the assembly of well-formed VLPs under a wide range of attraction strengths. These observations establish a protocol for the efficient in vitro assembly of CCMV VLPs and suggest potential strategies that the virus may employ in vivo.
page start: 1050
estado: published
related by: authorship_22075
issue: 5
doi: 10.1016/j.jmb.2013.10.017
related by: authorship_22076
page end: 1060

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CHE 1051507
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funding vehicle for: The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions

RDF description of The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions - http://orbis.uaslp.mx/vivo/individual/scopus3825
rights: termsOfUse
date: 2026-08-26T03:56:50
publisher: vivo
type: Document

Journal of Molecular Biology
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publication venue for: The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions
type: Generically Dependent Continuant
type: Document

National Center for Research Resources, NCRR: S10RR023057
type: Relationship
type: BFO_0000020
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type: Thing
type: Grant
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funding vehicle for: The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions

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Information Content Entity
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