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cowpea chlorotic mottle virus; packaging; self-assembly; ssRNA; virus-like particle capsid protein; single stranded RNA; virus RNA; article; cowpea; cowpea chlorotic mottle virus; electron microscopy; ionic strength; nonhuman; pH; priority journal; protein assembly; protein protein interaction; protein RNA binding; sedimentation rate; sucrose density gradient centrifugation; virus capsid; virus genome; virus like agent; virus nucleocapsid; Article; controlled study; cowpea chlorotic mottle virus; in vitro study; ionic strength; RNA virus; virus assembly; Cowpea chlorotic mottle virus; Miridae; RNA viruses; cowpea chlorotic mottle virus; packaging; self-assembly; ssRNA; virus-like particle; Bromovirus; Capsid Proteins; Microscopy, Electron; RNA, Viral; Virus Assembly
The strength of attraction between capsid proteins (CPs) of cowpea chlorotic mottle virus (CCMV) is controlled by the solution pH. Additionally, the strength of attraction between CP and the single-stranded RNA viral genome is controlled by ionic strength. By exploiting these properties, we are able to control and monitor the in vitro co-assembly of CCMV CP and single-stranded RNA as a function of the strength of CP-CP and CP-RNA attractions. Using the techniques of velocity sedimentation and electron microscopy, we find that the successful assembly of nuclease-resistant virus-like particles (VLPs) depends delicately on the strength of CP-CP attraction relative to CP-RNA attraction. If the attractions are too weak, the capsid cannot form; if they are too strong, the assembly suffers from kinetic traps. Separating the process into two steps - by first turning on CP-RNA attraction and then turning on CP-CP attraction - allows for the assembly of well-formed VLPs under a wide range of attraction strengths. These observations establish a protocol for the efficient in vitro assembly of CCMV VLPs and suggest potential strategies that the virus may employ in vivo.
The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions
Generically Dependent Continuant
RDF description of The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions - http://orbis.uaslp.mx/vivo/individual/scopus3825
National Center for Research Resources, NCRR: S10RR023057
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