Document
type: Class

Continuant
type: Class

Entity
type: Class

Generically Dependent Continuant
type: Class

Tohoku Journal of Experimental Medicine
type: Journal
type: Thing
type: Entity
type: Information Content Entity
publication venue for: A novel method for measuring the ATP-related compounds in human erythrocytes
type: Continuant
type: Generically Dependent Continuant
type: Periodical
type: Collection
type: Document

RDF description of A novel method for measuring the ATP-related compounds in human erythrocytes - http://orbis.uaslp.mx/vivo/individual/scopus3786
rights: termsOfUse
date: 2026-08-24T21:49:40
publisher: vivo
type: Document

Article
type: Class

A novel method for measuring the ATP-related compounds in human erythrocytes
type: Generically Dependent Continuant
issue: 3
related by: authorship_21854
page end: 214
has publication venue: Tohoku Journal of Experimental Medicine
volume: 233
freetext keyword: Adenosine triphosphate; Energetic homeostasis; Erythrocytes; Liquid chromatography; Whole blood sample hypoxanthine; inosine; inosine phosphate; uric acid; adenosine; adenosine triphosphate; hypoxanthine; inosine; inosine phosphate; uric acid; adult; analytic method; article; blood sampling; chronic kidney failure; diode; diode array detector; drug monitoring; environmental exposure; enzymatic degradation; erythrocyte; homeostasis; human; human cell; in vitro study; liquid chromatography; male; malignant neoplastic disease; normal human; organ preservation; chemistry; erythrocyte; erythrocyte count; high performance liquid chromatography; metabolism; pH; physiology; procedures; sensitivity and specificity; Adenosine; Adenosine Triphosphate; Chromatography, High Pressure Liquid; Erythrocyte Count; Erythrocytes; Homeostasis; Humans; Hydrogen-Ion Concentration; Hypoxanthine; Inosine; Inosine Monophosphate; Sensitivity and Specificity; Uric Acid
type: Information Content Entity
type: Thing
date time value: article date 3787
most specific type: Article
related by: authorship_21851
type: Document
doi: 10.1620/tjem.233.205
abstract: The ATP-related compounds in whole blood or red blood cells have been used to evaluate the energy status of erythrocytes and the degradation level of the phosphorylated compounds under various conditions, such as chronic renal failure, drug monitoring, cancer, exposure to environmental toxics, and organ preservation. The complete interpretation of the energetic homeostasis of erythrocytes is only performed using the compounds involved in the degradation pathway for adenine nucleotides alongside the uric acid value. For the first time, we report a liquid chromatographic method using a diode array detector that measures all of these compounds in a small human whole blood sample (125 μ L) within an acceptable time of 20 min. The stability was evaluated for all of the compounds and ranged from 96.3 to 105.1%25 versus the day zero values. The measurement had an adequate sensitivity for the ATP-related compounds (detection limits from 0.001 to 0.097 μ mol/L and quantification limits from 0.004 to 0.294 μ mol/L). This method is particularly useful for measuring inosine monophosphate, inosine, hypoxanthine, and uric acid. Moreover, this assay had acceptable linearity (r > 0.990), precision (coefficients of variation ranged from 0.1 to 2.0%25), specificity (similar retention times and spectra in all samples) and recoveries (ranged from 89.2 to 104.9%25). The newly developed method is invaluable for assessing the energetic homeostasis of red blood cells under diverse conditions, such as in vitro experiments and clinical settings. © 2014 Tohoku University Medical Press.
related by: authorship_21853
chemicalscas: hypoxanthine, 68-94-0; inosine, 58-63-9; inosine phosphate, 131-99-7; uric acid, 69-93-2; adenosine, 58-61-7; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; Adenosine; Adenosine Triphosphate; Hypoxanthine; Inosine; Inosine Monophosphate; Uric Acid
e iscopus: 2-s2.0-84904680484
type: Entity
type: Article
estado: published
origen: scopus
type: Continuant
accesstype: All Open Access, Bronze, Green
pmid: 25048613
most specific type: Document
related by: authorship_21852
crisid: 3787
page start: 205

Information Content Entity
type: Class