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Generically Dependent Continuant
Silver nanoparticles (AgNPs) are used to manufacture materials with new properties and functions. However, little is known about their toxic or beneficial effects on human health, especially in the respiratory system, where its smooth muscle (ASM) regulates the airway contractility by different mediators, such as acetylcholine (ACh) and nitric oxide (NO). The aim of this study was to evaluate the effects of AgNPs on ASM cells. Exposure to AgNPs induced ACh-independent expression of the inducible nitric oxide synthase (iNOS) at 100. μg/mL, associated with excessive production of NO. AgNPs induced the muscarinic receptor activation, since its blockage with atropine and blockage of its downstream signaling pathway inhibited the NO production. AgNPs at 10 and 100. μg/mL induced ACh-independent prolonged cytotoxicity and decreased cellular proliferation mediated by the muscarinic receptor-iNOS pathway. However, the concentration of 100. μg/mL of AgNPs induced muscarinic receptor-independent apoptosis, suggesting the activation of multiple pathways. These data indicate that AgNPs induce prolonged cytotoxic and anti-proliferative effects on ASM cells, suggesting an activation of the muscarinic receptor-iNOS pathway. Further investigation is required to understand the full mechanisms of action of AgNPs on ASM under specific biological conditions. © 2013 Elsevier Ireland Ltd.
Airway; Apoptosis; Muscarinic receptor; Nitric oxide; Proliferation; Silver nanoparticles acetylcholine; atropine; inducible nitric oxide synthase; muscarinic receptor; nitric oxide; silver nanoparticle; animal cell; antiproliferative activity; apoptosis; article; cell proliferation; clinical evaluation; controlled study; cytotoxicity; enzyme activation; male; muscle contractility; nonhuman; priority journal; protein expression; rat; respiratory system; signal transduction; smooth muscle; smooth muscle fiber; Airway; Apoptosis; Muscarinic receptor; Nitric oxide; Proliferation; Silver nanoparticles; Acetylcholine; Animals; Apoptosis; Cell Proliferation; Male; Metal Nanoparticles; Myocytes, Smooth Muscle; Nitric Oxide; Nitric Oxide Synthase Type II; Rats; Rats, Wistar; Receptors, Muscarinic; Signal Transduction; Silver; Trachea
acetylcholine, 51-84-3, 60-31-1, 66-23-9; atropine, 51-55-8, 55-48-1; inducible nitric oxide synthase, 501433-35-8; nitric oxide, 10102-43-9; Acetylcholine, N9YNS0M02X; Nitric Oxide, 31C4KY9ESH; Nitric Oxide Synthase Type II, 1.14.13.39; Receptors, Muscarinic; Silver, 3M4G523W1G
Silver nanoparticles induce anti-proliferative effects on airway smooth muscle cells. Role of nitric oxide and muscarinic receptor signaling pathway
RDF description of Silver nanoparticles induce anti-proliferative effects on airway smooth muscle cells. Role of nitric oxide and muscarinic receptor signaling pathway - http://orbis.uaslp.mx/vivo/individual/scopus3738
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