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RDF description of The Opuntia streptacantha OpsHSP18 gene confers salt and osmotic stress tolerance in Arabidopsis thaliana - http://orbis.uaslp.mx/vivo/individual/scopus3148
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Abiotic stress limits seed germination, plant growth, flowering and fruit quality, causing economic decrease. Small Heat Shock Proteins (sHSPs) are chaperons with roles in stress tolerance. Herein, we report the functional characterization of a cytosolic class CI sHSP (OpsHSP18) from Opuntia streptacantha during seed germination in Arabidopsis thaliana transgenic lines subjected to different stress and hormone treatments. The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT. On the other hand, the over-expression of the OpsHSP18 gene enhanced tolerance to salt (150 mM NaCl) and osmotic (274 mM mannitol) stress in Arabidopsis seedlings treated during 14 and 21 days, respectively. These plants showed increased survival rates (52.00 and 73.33%25, respectively) with respect to the WT (18.75 and 53.75%25, respectively). Thus, our results show that OpsHSP18 gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Abiotic stress; Abscisic acid; Arabidopsis thaliana; Germination; Opuntia streptacantha; Small heat shock protein glucose; mannitol; sodium chloride; abscisic acid; messenger RNA; phytohormone; small heat shock protein; sodium chloride; Arabidopsis thaliana; article; controlled study; gene overexpression; germination; HSP18 gene; nonhuman; nucleotide sequence; Opuntia; opuntia streptacantha; osmotic stress; plant gene; salt stress; salt tolerance; seedling; wild type; amino acid sequence; Arabidopsis; drug effects; genetics; growth, development and aging; metabolism; molecular genetics; osmotic pressure; phylogeny; physiological stress; real time polymerase chain reaction; reverse transcription polymerase chain reaction; sequence homology; Arabidopsis; Arabidopsis thaliana; Opuntia streptacantha; Abscisic Acid; Amino Acid Sequence; Arabidopsis; Germination; Heat-Shock Proteins, Small; Molecular Sequence Data; Opuntia; Osmotic Pressure; Phylogeny; Plant Growth Regulators; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Seedling; Sequence Homology, Amino Acid; Sodium Chloride; Stress, Physiological
The Opuntia streptacantha OpsHSP18 gene confers salt and osmotic stress tolerance in Arabidopsis thaliana
glucose, 50-99-7, 84778-64-3; mannitol, 69-65-8, 87-78-5; sodium chloride, 7647-14-5; abscisic acid, 21293-29-8; Abscisic Acid; Heat-Shock Proteins, Small; Plant Growth Regulators; RNA, Messenger; Sodium Chloride
International Journal of Molecular Sciences
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