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RDF description of Autocrine modulation of glucose transporter SGLT2 by IL-6 and TNF-α in LLC-PK1 cells - http://orbis.uaslp.mx/vivo/individual/scopus3015
Article
We determined in cultured kidney epithelial cells (LLC-PK1) the effects of high glucose, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) on mRNA and protein expression of the renal glucose transporters SGLT1 and SGLT2. Culturedmonolayers were incubated with similar concentrations of IL-6 and TNF-α to those produced by LLC-PK1 in the presence of 20mMglucose. Confluent monolayers with either 5 (controls, C) or 20 mM glucose (high glucose, HG) were incubated in the presence of 5 mM glucose, 20 mM glucose, 10 pg/ml IL-6, or TNF-α alone or in combination. Separate groups with IL-6 and TNF-α were incubated with antibodies to their respective receptors. HG induced an increased SGLT1 mRNA at 48 h (p<0.05 vs. C) and protein expression in 120 h (p<0.05 vs. C). HG also induced an increased SGLT2 mRNA at 72 and 96 h (P<0.05 vs. C) and SGLT2 protein expression at 120 h (p<0.05 vs. C). In C, 10 pg/ml IL-6 or TNF-α did not modify SGLT1 mRNA (n.s vs. in the absence of cytokines). In contrast, cytokines induced an increased expression of SGLT1 protein at 120 h (p<0.05 vs. in the absence of cytokines), and SGLT2 mRNA and protein were increased at 96 and 120 h, respectively (p<0.05 vs. in absence of cytokines). No changes were observed when cells were incubated with cytokines and HG (n.s vs. C). In conclusion, this study showed that SGLT2 increased in the presence of IL-6 and TNF-α, indicating an autocrine modulation of the expression of this transporter by cytokines. © University of Navarra 2012.
Glucose Transport Proteins, Facilitative; Interleukin-6; RNA, Messenger; Sodium-Glucose Transporter 1; Sodium-Glucose Transporter 2; Tumor Necrosis Factor-alpha
Glucose; IL-6; SGLT2; SGLTI; TNF-α interleukin 6; messenger RNA; sodium glucose cotransporter 1; sodium glucose cotransporter 2; tumor necrosis factor alpha; animal cell; article; autocrine effect; gene expression; kidney cell culture; kidney epithelium; nonhuman; protein expression; Animals; Cells, Cultured; Glucose Transport Proteins, Facilitative; Interleukin-6; RNA, Messenger; Sodium-Glucose Transporter 1; Sodium-Glucose Transporter 2; Swine; Tumor Necrosis Factor-alpha
Autocrine modulation of glucose transporter SGLT2 by IL-6 and TNF-α in LLC-PK1 cells
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