Consejo Nacional de Ciencia y Tecnología, CONACYT: 202061
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota)

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type: Class

Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota)
has funding vehicle: Consejo Nacional de Ciencia y Tecnología, CONACYT: 202061
volume: 72
abstract: While a long shelf life for fruit products is highly desired, enzymatic browning is the main cause of quality loss in fruits and is therefore a main problem for the food industry. In this study polyphenol oxidase (PPO), the main enzyme responsible for browning was isolated from mamey fruit (Pouteria sapota) and characterized biochemically. Two isoenzymes (PPO 1 and PPO 2) were obtained upon ammonium sulfate precipitation and hydrophobic and ion exchange chromatography; PPO 1 was purified up to 6.6-fold with 0.28%25 yield, while PPO 2 could not be characterized as enzyme activity was completely lost after 24 h of storage. PPO 1 molecular weight was estimated to be 16.1 and 18 kDa by gel filtration and SDS-PAGE, respectively, indicating that the native state of the PPO 1 is a monomer. The optimum pH for PPO 1 activity was 7. The PPO 1 was determined to be maximum thermally stable up to 35 °C. Kinetic constants for PPO 1 were Km = 44 mM and Km = 1.3 mM using catechol and pyrogallol as substrate, respectively. The best substrates for PPO 1 were pyrogallol, 4-methylcatechol and catechol, while ascorbic acid and sodium metabisulfite were the most effective inhibitors. © 2010 Elsevier Ltd. All rights reserved.
origen: scopus
related by: authorship_15513
type: Entity
type: Document
related by: authorship_15512
type: Article
doi: 10.1016/j.phytochem.2010.10.011
has funding vehicle: Instituto Politécnico Nacional, IPN
most specific type: Article
type: Continuant
related by: authorship_15514
page end: 88
related by: authorship_15511
pmid: 21087780
has publication venue: Phytochemistry
issue: 1
crisid: 2642
chemicalscas: 4 methylcatechol, 452-86-8; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; pyrogallol, 87-66-1; sodium metabisulfite, 7681-57-4, 7757-74-6; sulfite, 14265-45-3; 4-methylcatechol, 452-86-8; Ascorbic Acid, 50-81-7; Catechol Oxidase, 1.10.3.1; Catechols; Isoenzymes; Pyrogallol, 87-66-1; Sulfites; sodium metabisulfite, 7681-57-4
type: Thing
type: Generically Dependent Continuant
page start: 82
type: Information Content Entity
related by: authorship_15515
estado: published
date time value: article date 2642
e iscopus: 2-s2.0-78650712936
most specific type: Document
freetext keyword: Catechol; Polyphenol oxidase; PPO purification; Pyrogallol; Sapotaceae; Sapote mamey (Pouteria sapota) 4 methylcatechol; 4-methylcatechol; ascorbic acid; catechol derivative; catechol oxidase; isoenzyme; pyrogallol; sodium metabisulfite; sulfite; article; drug antagonism; enzymology; fruit; ion exchange chromatography; isolation and purification; metabolism; Mexico; molecular weight; pH; polyacrylamide gel electrophoresis; Pouteria; thermodynamics; Ascorbic Acid; Catechol Oxidase; Catechols; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Fruit; Hydrogen-Ion Concentration; Isoenzymes; Mexico; Molecular Weight; Pouteria; Pyrogallol; Sulfites; Thermodynamics; Casimiroa; Mammea americana; Pouteria; Pouteria sapota; Sapotaceae

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RDF description of Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota) - http://orbis.uaslp.mx/vivo/individual/scopus2641
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date: 2026-08-27T06:56:03
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Article
type: Class

Phytochemistry
publication venue for: Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota)
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type: Information Content Entity
type: Continuant
type: Document
type: Periodical
type: Journal
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Instituto Politécnico Nacional, IPN
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota)