Document
type: Class

Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen
freetext keyword: Biochemistry; Chemistry; Amplification curves; Amplification products; Fluorochromes; Human papillomavirus; Neoplastic lesions; Quantitative PCR; Thermal denaturations; Viral load; Polymerase chain reaction; evagreen; fluorochrome; article; denaturation; gene sequence; Human papillomavirus type 16; nonhuman; nucleotide sequence; oncogene; polymerase chain reaction; priority journal; quantitative polymerase chain reaction; real time polymerase chain reaction; virus detection; Human papillomavirus - 16; Human papillomavirus type 16
abstract: Quantitation of E6 oncogene sequences of the human papillomavirus type 16 by real-time or quantitative PCR (qPCR) is used to determine the viral load, which correlates with the degree of the cervical neoplastic lesions. In the presence of EvaGreen, a new DNA intercalating fluorochrome, we obtained consistent and reproducible qPCR amplification curves and thermal denaturation profiles identical to those of the authentic E6-HPV16 (human papillomavirus 16) genome from the amplification products derived from a construct carrying the E6-HPV16 oncogene. E6-HPV16 quantitation in the presence of EvaGreen, therefore, is reproducible and specific and may be used to determine HPV16 viral load. © 2008 Elsevier Inc. All rights reserved.
has funding vehicle: Consejo Nacional de Ciencia y Tecnología, CONACYT: FMSLP-C01-2006-62319
related by: authorship_11501
doi: 10.1016/j.ab.2008.05.012
page end: 133
pmid: 18539127
issue: 1
date time value: article date 1937
type: Entity
type: Continuant
most specific type: Article
type: Information Content Entity
volume: 380
e iscopus: 2-s2.0-46649094359
page start: 131
type: Document
estado: published
type: Generically Dependent Continuant
most specific type: Document
type: Thing
chemicalscas: fluorochrome, 37332-03-9
crisid: 1937
molecularseqnum: GENBANK: NC_001526
has publication venue: Analytical Biochemistry
origen: scopus
related by: authorship_11500
has funding vehicle: 176112
type: Article

176112
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen

Consejo Nacional de Ciencia y Tecnología, CONACYT: FMSLP-C01-2006-62319
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen

Continuant
type: Class

Entity
type: Class

Analytical Biochemistry
type: Continuant
type: Entity
type: Document
type: Information Content Entity
publication venue for: Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen
type: Thing
type: Generically Dependent Continuant
type: Periodical
type: Journal
type: Collection

Generically Dependent Continuant
type: Class

RDF description of Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen - http://orbis.uaslp.mx/vivo/individual/scopus1936
rights: termsOfUse
date: 2026-08-26T10:50:48
publisher: vivo
type: Document

Article
type: Class

Information Content Entity
type: Class