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U.S. Public Health Service, USPHS
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funding vehicle for: Highly sensitive immunoadsorption procedure for detection of low-abundance proteins

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Analytical Biochemistry
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publication venue for: Highly sensitive immunoadsorption procedure for detection of low-abundance proteins
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National Science Foundation, NSF: CA05388, PCM-835 1884
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funding vehicle for: Highly sensitive immunoadsorption procedure for detection of low-abundance proteins

RDF description of Highly sensitive immunoadsorption procedure for detection of low-abundance proteins - http://orbis.uaslp.mx/vivo/individual/scopus193
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date: 2026-08-25T07:34:59
publisher: vivo
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National Cancer Institute, NCI: P01CA005388, R01CA035547
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funding vehicle for: Highly sensitive immunoadsorption procedure for detection of low-abundance proteins

Article
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Highly sensitive immunoadsorption procedure for detection of low-abundance proteins
type: Article
page end: 135
estado: published
most specific type: Article
page start: 126
type: Document
chemicalscas: dodecyl sulfate sodium, 151-21-3; protein, 67254-75-5; staphylococcus protein A, 37380-84-0; Proteins; Sodium Dodecyl Sulfate, 151-21-3; Staphylococcal Protein A; Viral Proteins
freetext keyword: critical micelle concentration; fluorography; low-abundance proteins; protein A; sodium dodecyl sulfate; staph A immunoadsorption dodecyl sulfate sodium; protein; staphylococcus protein A; virus protein; protein; article; chemistry; enzyme linked immunosorbent assay; micelle; Mouse mammary tumor oncovirus; precipitation; solubility; temperature; antigen antibody complex; immunoadsorption; methodology; micelle; nonhuman; priority journal; Immunosorbent Techniques; Mammary Tumor Virus, Mouse; Micelles; Microchemistry; Precipitation; Proteins; Sodium Dodecyl Sulfate; Solubility; Staphylococcal Protein A; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.; Support, U.S. Gov't, P.H.S.; Temperature; Viral Proteins; mammary tumor virus; Mouse mammary tumor virus; tumor virus
related by: authorship 891
type: Generically Dependent Continuant
pmid: 3017145
has funding vehicle: National Science Foundation, NSF: CA05388, PCM-835 1884
related by: authorship 890
type: Entity
has funding vehicle: National Cancer Institute, NCI: P01CA005388, R01CA035547
has funding vehicle: U.S. Public Health Service, USPHS
date time value: article date 194
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crisid: 194
related by: authorship 889
type: Information Content Entity
doi: 10.1016/0003-2697(86)90163-6
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e iscopus: 2-s2.0-0022555675
most specific type: Document
has publication venue: Analytical Biochemistry
issue: 1
volume: 156
related by: authorship 892
origen: scopus
related by: authorship 888
abstract: A procedure that virtually eliminates nonspecific adsorption of radiolabeled proteins during immunoprecipitation was devised utilizing staphylococcal cells containing protein A (Staph A). Immunoprecipitates (antigen-antibody complexes) were solubilized from Staph A pellets into detergent micelles by incubation in a small volume of 1%25 sodium dodecyl sulfate (SDS) at 23°C for 10 min. To allow re-formation of immunocomplexes and rebinding to new Staph A, the SDS-solubilized material was diluted 20-fold in buffer containing 1%25 Triton X-100 and 0.5%25 sodium deoxycholate. Specific conductance measurements revealed that this solubilization and subsequent reimmunoadsorption of antibody-antigen complexes occur at SDS concentrations that are first above and then below its critical micelle concentration. This procedure lowered the nonspecific background from approximately 2250 parts per million (ppm) to less than 25 ppm with a final recovery of 30-50%25 depending on the antigen and antibody. Chaotropic agents such as 2 m urea, 0.2 m KOH, and 3.5 m MgCl2 (as well as combinations of urea and SDS) can substitute for 1%25 SDS, although the final recovery is somewhat lower. Fluorography of radiolabeled proteins obtained in this manner displays virtually undetectable background even for exposures as long as 2 months. These methods allowed the unambiguous detection of low-abundance antigens at a high level of sensitivity, for example, mouse mammary tumor virus protein products and epidermal growth factor receptor. © 1986.

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