Document
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Consejo Nacional de Ciencia y Tecnología, CONACYT: 165946, 172307
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funding vehicle for: Transgenic tomatoes express an antigenic polypeptide containing epitopes of the diphtheria, pertussis and tetanus exotoxins, encoded by a synthetic gene

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individual
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date: 2026-08-28T03:37:39
publisher: vivo
type: Document
label: RDF description of Transgenic tomatoes express an antigenic polypeptide containing epitopes of the diphtheria, pertussis and tetanus exotoxins, encoded by a synthetic gene - http://orbis.uaslp.mx/vivo/individual/scopus1850

Article
type: Class

Plant Cell Reports
publication venue for: Transgenic tomatoes express an antigenic polypeptide containing epitopes of the diphtheria, pertussis and tetanus exotoxins, encoded by a synthetic gene
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Transgenic tomatoes express an antigenic polypeptide containing epitopes of the diphtheria, pertussis and tetanus exotoxins, encoded by a synthetic gene
type: Thing
page start: 961
chemicalscas: pertussis toxin, 70323-44-3; Antigens, Bacterial; Diphtheria Toxin; Epitopes; Pertussis Toxin, EC 2.4.2.31; Tetanus Toxin
type: Entity
most specific type: Document
pmid: 17619922
volume: 26
type: Information Content Entity
type: Article
has publication venue: Plant Cell Reports
type: Generically Dependent Continuant
most specific type: Article
e iscopus: 2-s2.0-34548431801
related by: authorship_11087
type: Continuant
page end: 968
related by: authorship_11086
crisid: 1851
type: Document
related by: authorship_11091
related by: authorship_11089
has funding vehicle: Consejo Nacional de Ciencia y Tecnología, CONACYT: 165946, 172307
related by: authorship_11088
abstract: A current priority of vaccinology is the development of multicomponent vaccines that protect against several pathogens. The diphtheria-pertussis- tetanus (DPT) vaccine prevents the symptoms of three serious and often fatal diseases due to the exotoxins produced by Corynebacterium diphteriae, Bordetella pertussis and Clostridium tetani. We are attempting to develop an edible DPT multicomponent vaccine in plants, based on the fusion of protective exotoxin epitopes encoded by synthetic genes. By means of Agrobacterium mediated transformation we generated transgenic tomatoes with a plant-optimised synthetic gene encoding a novel polypeptide containing two adjuvant and six DPT immunoprotective exotoxin epitopes joined by peptide linkers. In transformed tomato plants, integration of the synthetic DPT (sDPT) gene detected by PCR was confirmed by Southern blot, and specific transcripts of the expected molecular size were detected by RT-PCR. Expression of the putative polypeptide encoded by the sDPT gene was detected by immunoassay with specific antibodies to the diphtheria, pertussis and tetanus exotoxins. The sDPT gene is therefore integrated, transcribed and translated as the expected recombinant sDPT multiepitope polypeptide in transgenic tomatoes that constitute a potential edible vaccine. © 2007 Springer-Verlag.
freetext keyword: Diphtheria; Synthetic genes; Tetanus; Transgenic plants; Whooping cough Biosynthesis; Enzyme kinetics; Gene expression; Pathogens; Polypeptides; Vaccines; Corynebacterium diphteriae; Synthetic genes; Transgenic plants; Whooping cough; Fruits; Agrobacterium; Bordetella pertussis; Clostridium tetani; Corynebacterium; Lycopersicon esculentum; Rhizobium; bacterial antigen; diphtheria toxin; epitope; pertussis toxin; tetanus toxin; amino acid sequence; article; chemistry; gene expression regulation; genetics; metabolism; molecular cloning; tomato; transgenic plant; Amino Acid Sequence; Antigens, Bacterial; Cloning, Molecular; Diphtheria Toxin; Epitopes; Gene Expression Regulation, Plant; Lycopersicon esculentum; Pertussis Toxin; Plants, Genetically Modified; Tetanus Toxin
origen: scopus
date time value: article date 1851
related by: authorship_11090
doi: 10.1007/s00299-007-0306-y
issue: 7
estado: published