Is there still room for novel viral pathogens in pediatric respiratory tract infections?
type: Article
related by: authorship_11009
related by: authorship_10996
related by: authorship_11003
related by: authorship_11001
doi: 10.1371/journal.pone.0113570
freetext keyword: virus DNA; DNA virus; virus DNA; virus RNA; Acidovorax; Acinetobacter baumannii; Actinomycetales; Adenovirus; adolescent; Anellovirus; animal virus; Article; Astrovirus; bacterium; Bocavirus; Burkholderia cepacia; Burkholderia gladioli; child; controlled study; Coronavirus; disease association; DNA library; DNA sequence; Enterovirus; female; Haemophilus influenzae; hospitalization; human; infant; Influenza virus A; Influenza virus B; Klebsiella pneumoniae; Legionella pneumophila; Leifsonia xyli; lower respiratory tract infection; major clinical study; Malassezia globosa; male; metagenomics; Metapneumovirus; Moraxella catarrhalis; Mycobacterium tuberculosis; nasal washing; nasopharyngeal swab; nonhuman; outpatient; Parainfluenza virus 1; Parainfluenza virus 2; Parainfluenza virus 3; Parainfluenza virus 4; pathogenesis; pediatrics; personal hygiene; phylogeny; plant virus; pneumonia; polymerase chain reaction; preschool child; Pseudomonas aeruginosa; Pseudomonas mendocina; Rahnella; Respiratory syncytial pneumovirus; respiratory tract examination; respiratory virus; reverse transcription polymerase chain reaction; Rhinovirus; Rotavirus; Scaffold virus; school child; sequence analysis; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus pneumoniae; throat culture; upper respiratory tract infection; virus detection; virus identification; virus strain; classification; Coronaviridae; genetics; hospitalized child; Human respiratory syncytial virus; nasopharynx; pathology; physiology; respiratory tract infection; RNA virus; virology; Child; Child, Hospitalized; Child, Preschool; Coronavirus; DNA Viruses; DNA, Viral; Female; Humans; Infant; Male; Nasopharynx; Phylogeny; Pneumonia; Respiratory Syncytial Viruses; Respiratory Tract Infections; Rhinovirus; RNA Viruses; RNA, Viral; Sequence Analysis, DNA; Sequence Analysis, RNA
related by: authorship_10997
related by: authorship_11004
type: Document
artnum: e113570
related by: authorship_10994
related by: authorship_10999
volume: 9
related by: authorship_11006
type: Information Content Entity
estado: published
e iscopus: 2-s2.0-84911913249
type: Continuant
most specific type: Article
chemicalscas: DNA, Viral; RNA, Viral
related by: authorship_10998
related by: authorship_11005
related by: authorship_10991
related by: authorship_10989
crisid: 1834
has publication venue: PLoS ONE
accesstype: All Open Access, Gold, Green
related by: authorship_11007
related by: authorship_10990
type: Entity
related by: authorship_11008
related by: authorship_10995
related by: authorship_11002
related by: authorship_11000
type: Generically Dependent Continuant
most specific type: Document
abstract: Viruses are the most frequent cause of respiratory disease in children. However, despite the advanced diagnostic methods currently in use, in 20 to 50%25 of respiratory samples a specific pathogen cannot be detected. In this work, we used a metagenomic approach and deep sequencing to examine respiratory samples from children with lower and upper respiratory tract infections that had been previously found negative for 6 bacteria and 15 respiratory viruses by PCR. Nasal washings from 25 children (out of 250) hospitalized with a diagnosis of pneumonia and nasopharyngeal swabs from 46 outpatient children (out of 526) were studied. DNA reads for at least one virus commonly associated to respiratory infections was found in 20 of 25 hospitalized patients, while reads for pathogenic respiratory bacteria were detected in the remaining 5 children. For outpatients, all the samples were pooled into 25 DNA libraries for sequencing. In this case, in 22 of the 25 sequenced libraries at least one respiratory virus was identified, while in all other, but one, pathogenic bacteria were detected. In both patient groups reads for respiratory syncytial virus, coronavirus-OC43, and rhinovirus were identified. In addition, viruses less frequently associated to respiratory infections were also found. Saffold virus was detected in outpatient but not in hospitalized children. Anellovirus, rotavirus, and astrovirus, as well as several animal and plant viruses were detected in both groups. No novel viruses were identified. Adding up the deep sequencing results to the PCR data, 79.2%25 of 250 hospitalized and 76.6%25 of 526 ambulatory patients were positive for viruses, and all other children, but one, had pathogenic respiratory bacteria identified. These results suggest that at least in the type of populations studied and with the sampling methods used the odds of finding novel, clinically relevant viruses, in pediatric respiratory infections are low. © 2014 Taboada et al.
origen: scopus
issue: 11
related by: authorship_10992
pmid: 25412469
related by: authorship_10993
type: Thing
date time value: article date 1834

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RDF description of Is there still room for novel viral pathogens in pediatric respiratory tract infections? - http://orbis.uaslp.mx/vivo/individual/scopus1833
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date: 2026-08-24T21:51:05
publisher: vivo
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PLoS ONE
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