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Phenotypic analysis of IL-10-treated, monocyte-derived dendritic cells in patients with systemic lupus erythematosus
fibronectin, 86088-83-7; interleukin 12, 138415-13-1; Antigens, CD; HLA-DR Antigens; Interleukin-10, 130068-27-8; Receptors, Cell Surface
B7 antigen; CD11b antigen; cd151 antigen; CD69 antigen; CD85j antigen; CD86 antigen; CD9 antigen; fibronectin; fibronectin 1; HLA DR antigen; interleukin 10; interleukin 12; lymphocyte antigen; major histocompatibility antigen class 2; tetraspanin; unclassified drug; adult; antigen expression; article; blood sampling; cell isolation; clinical article; controlled study; dendritic cell; female; human; human cell; immunological tolerance; in vitro study; male; monocyte; phenotype; priority journal; protein synthesis; quantitative analysis; systemic lupus erythematosus; Adult; Antigens, CD; Cell Differentiation; Dendritic Cells; Female; Flow Cytometry; HLA-DR Antigens; Humans; Interleukin-10; Lupus Erythematosus, Systemic; Male; Middle Aged; Monocytes; Phenotype; Receptors, Cell Surface
Dendritic cells (DC) play a dual role in the immune response, participating in its induction, and the maintenance of immune tolerance. The aim of this work was to perform a quantitative and phenotypic analysis of DC generated in vitro in the presence of IL-10 in patients with systemic lupus erythematosus (SLE). Blood samples were obtained from 10 active and untreated patients with SLE and six controls. Monocyte-derived DC were generated in vitro in the presence or absence of IL-10, and a quantitative and phenotypic analysis was performed. We found that freshly isolated monocytes from SLE patients had an increased expression of CD11b. On the other hand, the efficiency of in vitro DC generation was diminished in blood samples from SLE patients for conventional DC, but not for IL-10-treated DC. A diminished expression of HLA-DR, CD9 and CD86 was observed in conventional DC from SLE patients compared with controls. In contrast, enhanced levels of HLA-DR, CD80, CD9 and CD151 tetraspanins, FN1 (a class II MHC-tetraspanin epitope), CD85j/ILT2 and CD69 were detected in IL-10-treated DC from SLE patients. Accordingly, the phenotypic profile of IL-10-treated DC was very different in SLE and controls. However, the synthesis of IL-10 and IL-12 was similar in IL-10-treated and conventional cells in both SLE patients and controls. Our findings on the aberrant phenotype of IL-10-treated DC in SLE and their normal efficiency of in vitro generation may be important for the design of future therapies of this condition based on the administration of DC to induce immune tolerance. © 2006 The Authors.
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