Document
type: Class

Environmental Research
type: Generically Dependent Continuant
type: Collection
type: Entity
type: Periodical
type: Thing
type: Journal
type: Continuant
publication venue for: DDT induces apoptosis in human mononuclear cells in vitro and is associated with increased apoptosis in exposed children
type: Document
type: Information Content Entity

Continuant
type: Class

Entity
type: Class

Generically Dependent Continuant
type: Class

RDF description of DDT induces apoptosis in human mononuclear cells in vitro and is associated with increased apoptosis in exposed children - http://orbis.uaslp.mx/vivo/individual/scopus1297
rights: termsOfUse
date: 2026-08-24T21:51:08
publisher: vivo
type: Document

Article
type: Class

DDT induces apoptosis in human mononuclear cells in vitro and is associated with increased apoptosis in exposed children
crisid: 1298
related by: authorship 7632
type: Information Content Entity
type: Article
e iscopus: 2-s2.0-0344982948
related by: authorship 7633
volume: 94
pmid: 14643285
abstract: The aim of the present work was to investigate whether DDT and its metabolites are able to induce apoptosis of human peripheral blood mononuclear cells (PBMC) both in vitro and in vivo. Cells isolated from healthy individuals were incubated in the presence of increasing concentrations of p′p-DDT, p′p-DDE, or p′p-DDD (0-150μg/mL) for different intervals. Apoptosis was then determined by flow cytometry (DNA cell content analysis) and fluorescence microscopy (Hoechst staining). A significant level of apoptosis was induced by DDT, DDD, and DDE at 80μg/mL compared to controls, reaching a maximum effect at 100μg/mL. We began to detect apoptosis at 12h, with a maximum effect at 24h of incubation. These results were confirmed using the TUNEL assay in cells treated with the three compounds tested as well as with o′p-DDT at 100μg/mL and 24h of incubation. Our data demonstrate that DDT and its metabolites are able to induce apoptosis of human PBMC in vitro. Therefore, we performed a preliminary study in children exposed to this insecticide. When compared to a control population, the exposed children had higher levels of DDT, DDD, and DDE in blood and also had a higher frequency of apoptosis. In the exposed children, a weak positive association was found between the frequency of apoptosis and the exposure to DDT and DDE. Our results showed that more studies are needed in people exposed to DDT, as apoptosis may cause serious public health effects such as immunosuppression. © 2003 Elsevier Inc. All rights reserved.
has publication venue: Environmental Research
page start: 38
type: Thing
type: Continuant
has funding vehicle: 28432M
related by: authorship 7629
type: Document
origen: scopus
freetext keyword: Apoptosis; Children; DDE; DDT; Malaria 1,1 dichloro 2,2 bis(4 chlorophenyl)ethane; 1,1 dichloro 2,2 bis(4 chlorophenyl)ethylene; chlorphenotane; DNA; child; DDT; health impact; pesticide; pollution exposure; adult; apoptosis; article; blood level; cell isolation; child; concentration response; controlled study; cytopathogenic effect; DNA content; flow cytometry; fluorescence microscopy; genotoxicity; human; incubation time; mononuclear cell; nick end labeling; normal human; priority journal
most specific type: Document
issue: 1
page end: 46
estado: published
most specific type: Article
date time value: article date 1298
type: Entity
type: Generically Dependent Continuant
related by: authorship 7628
chemicalscas: 1,1 dichloro 2,2 bis(4 chlorophenyl)ethane, 72-54-8; 1,1 dichloro 2,2 bis(4 chlorophenyl)ethylene, 72-55-9; chlorphenotane, 50-29-3; DNA, 9007-49-2
doi: 10.1016/S0013-9351(03)00112-9
related by: authorship 7630
has funding vehicle: Commission for Environmental Cooperation, CEC: 24102461.037
related by: authorship 7631

Information Content Entity
type: Class

Commission for Environmental Cooperation, CEC: 24102461.037
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: DDT induces apoptosis in human mononuclear cells in vitro and is associated with increased apoptosis in exposed children

28432M
type: Relationship
type: BFO_0000020
type: Entity
type: Thing
type: Grant
type: Continuant
funding vehicle for: DDT induces apoptosis in human mononuclear cells in vitro and is associated with increased apoptosis in exposed children