Document
type: Class

Expression of functionally active α4β1 integrin by thymic epithelial cells
volume: 106
has publication venue: Clinical and Experimental Immunology
related by: authorship 589
related by: authorship 586
type: Generically Dependent Continuant
related by: authorship 593
related by: authorship 590
related by: authorship 596
pmid: 8870716
type: Article
most specific type: Article
type: Document
type: Thing
related by: authorship 587
page end: 178
related by: authorship 591
freetext keyword: 4 integrins; Epithelial cells; Human thymus beta1 integrin; integrin; vascular cell adhesion molecule 1; article; epithelium cell; fetus; flow cytometry; human; human cell; human tissue; normal human; priority journal; thymocyte; thymus; Animals; Cell Line; Epithelium; Humans; Infant; Integrin alpha4beta1; Integrins; Rats; Receptors, Lymphocyte Homing; Staining and Labeling; Thymus Gland
abstract: We have investigated the expression and function of the VLA-4 heterodimer α4β1, a member of the β1 integrin subfamily, on human thymic epithelial cells (TEC) derived from cortical epithelium. The expression of the α4 integrin chain was studied in four different cloned TEC lines derived from either fetal or post-natal human thymus by both flow cytometry and immunoprecipitation techniques with anti-α4 MoAbs. All different cell lines assayed expressed significant levels of α4, as revealed by their reactivity with MoAbs specific for distinct α4 epitopes. The α4 subunit expressed by TEC was associated to β1 but not to β7 chain, and displayed the characteristic 80/70 kD pattern of proteolytic cleavage. The VLA-4 integrin in these cells was constitutively active in terms of adhesiveness to both fibronectin and vascular cell adhesion molecule-1 (VCAM-1). In addition, this heterodimer localized to punctate regions of the cell in the area of contact with the substratum, named point contacts assessed by staining with the anti-β1 activation epitope 15/7 MoAb. According to the cortical origin of the TEC lines expressing VLA-4, human thymus sections stained with different anti-α4 antibodies revealed the presence of cortical, and in smaller numbers medullary epithelial cells bearing α4 integrin. The expression of α4 in the thymus was also found in both adult and fetal rats, in which epithelial cells were also specifically stained. Altogether, our data show that VLA-4 is an additional component of the integrin repertoire of TEC, and suggest that it could have an important role in thymus epithelial cell-thymocyte interactions.
related by: authorship 594
page start: 170
estado: published
origen: scopus
related by: authorship 588
type: Information Content Entity
crisid: 107
e iscopus: 2-s2.0-10144237202
related by: authorship 592
date time value: article date 107
most specific type: Document
related by: authorship 595
type: Continuant
issue: 1
chemicalscas: Integrin alpha4beta1; Integrins; Receptors, Lymphocyte Homing
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Continuant
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Entity
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Generically Dependent Continuant
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RDF description of Expression of functionally active α4β1 integrin by thymic epithelial cells - http://orbis.uaslp.mx/vivo/individual/scopus106
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date: 2026-08-24T21:51:13
publisher: vivo
type: Document

Article
type: Class

Information Content Entity
type: Class

Clinical and Experimental Immunology
publication venue for: Expression of functionally active α4β1 integrin by thymic epithelial cells
type: Journal
type: Collection
type: Thing
type: Information Content Entity
type: Generically Dependent Continuant
type: Entity
type: Periodical
type: Continuant
type: Document