Document
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Bacteria associated with apical periodontitis promotes in vitro the differentiation of macrophages to osteoclasts
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related by: authorship_71893
date time value: article date 10961
estado: published
doi: 10.1007/s00784-023-04920-8
related by: authorship_71892
pmid: 36800026
type: Entity
type: Document
abstract: To analyze the possible in vitro effect of the cytokine RANKL and bacteria involved in apical periodontitis on the differentiation of macrophages into osteoclasts. Material and methods: Bacteria were isolated (mainly E. faecium and E. faecalis) from the root canal of fifty patients with apical periodontitis, the possible effect of these bacteria on the phagocytic activity of the monocyte cell line THP-1 was analyzed by flow cytometry. Furthermore, the effect of these bacteria (alone or in combination with the cytokine RANKL) on the differentiation of THP-1 macrophages into osteoclasts was analyzed through the expression of the receptor RANK and the tartrate-resistant acid phosphatase TRAP. Finally, the release of different cytokines (IL-1β, TNF-α, IL-6, IL-8, IL-10, and IL-12p70) by THP-1 cells induced to differentiate into osteoclasts was also analyzed. Results: We observed a significant proportion of THP-1 cells were able to internalize E. faecium and E. faecalis. Furthermore, these bacteria were able to induce (alone or in combination with RANKL) a significant expression of RANK by THP-1 macrophages; accordingly, E. faecium and E. faecalis induced very significant levels of TRAP in these cells. Finally, during the differentiation of THP-1 macrophages induced by RANKL or bacteria, a significant release of the pro-inflammatory cytokines IL-6 and TNF-α was observed. Conclusions and clinical relevance: Our data suggest that the causative agents of apical periodontitis can induce the differentiation of osteoclasts as well as the release of pro-inflammatory cytokines, phenomena that may have an important role in the bone damage observed in this condition. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
has funding vehicle: CONACYT fellowship; Research Center for Health Sciences and Biomedicine, Autonomous University of San Luis Potosi; Endodontic Postgraduate Program of the Autonomous University of San Luis Potosi
related by: authorship_71896
crisid: 10961
type: Generically Dependent Continuant
type: Continuant
type: Information Content Entity
has publication venue: Clinical Oral Investigations
related by: authorship_71897
related by: authorship_71895
most specific type: Document
issue: 6
origen: scopus
volume: 27
page start: 3139
most specific type: Article
type: Article
freetext keyword: Apical periodontitis, Bone loss, E. faecalis, Osteoclasts, RANK, RANKL
page end: 3148
related by: authorship_71894

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RDF description of Bacteria associated with apical periodontitis promotes in vitro the differentiation of macrophages to osteoclasts - http://orbis.uaslp.mx/vivo/individual/d10960
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date: 2026-08-24T21:51:29
publisher: vivo
type: Document

Article
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CONACYT fellowship; Research Center for Health Sciences and Biomedicine, Autonomous University of San Luis Potosi; Endodontic Postgraduate Program of the Autonomous University of San Luis Potosi
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funding vehicle for: Bacteria associated with apical periodontitis promotes in vitro the differentiation of macrophages to osteoclasts

Information Content Entity
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Clinical Oral Investigations
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publication venue for: Bacteria associated with apical periodontitis promotes in vitro the differentiation of macrophages to osteoclasts
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