abstract

• The genera Bacillus belongs to the group of microorganisms that are known as plant growth-promoting bacteria, their metabolism has evolved to produce molecules that benefit the growth of the plant, and the production of 3-indole acetic acid (IAA) is part of its secondary metabolism. In this work, Bacillus subtilis was cultivated in a bioreactor to produce IAA using propionate and glucose as carbon sources in an M9-modified media; in both cases, tryptophan was added as a co-substrate. The yield of IAA using propionate is 17%25 higher compared to glucose. After 48 h of cultivation, the final concentration was 310 mg IAA/L using propionate and 230 mg IAA/L using glucose, with a concentration of 500 mg Trp/L. To gain more insight into propionate metabolism and its advantages, the genome-scale metabolic model of B. subtilis (iBSU 1147) and computational analysis were used to calculate flux distribution and evaluate the metabolic capabilities to produce IAA using propionate. The metabolic fluxes demonstrate that propionate uptake favors the production of precursors needed for the synthesis of the hormone, and the sensitivity analysis shows that the control of a specific growth rate has a positive impact on the production of IAA. © 2022 by the authors.

authors

• Rosales Colunga Luis Manuel

publication date

• 2022-01-01

funding provided via

• A1-S-30750, CVU 1109377; Consejo Nacional de Ciencia y Tecnología, CONACYT: CVU 943715  Grant

published in

• Microorganisms  Journal

keywords

• 3-indole acetic acid; Bacillus subtilis; flux balance analysis; propionate metabolism

Digital Object Identifier (DOI)

• 10.3390/microorganisms10122352

PubMed ID

start page

end page

volume

• 10

issue

• 12