abstract

• In the present work the temperature response of the constitutive S1 segment of the SARS-CoV-2 Spike Glycoprotein (GPS) has been studied. The intensity of the Raman bands remained almost constant before reaching a temperature of 133 °C. At this temperature a significant reduction of peak intensities was observed. Above 144 °C the spectra ceased to show any recognizable feature as that of the GPS S1, indicating that it had transformed after the denaturation process that it was subjected. The GPS S1 change is irreversible. Hence, Raman Spectroscopy (RS) provides a precision method to determine the denaturation temperature (TD) of dry powder GPS S1. The ability of RS was calibrated through the reproduction of TD of other well studied proteins as well as those of the decomposition temperature of some amino acids (AA). Through this study we established a TD of 139 ± 3 °C for powder GPS S1 of SARS-CoV-2. © 2021 Elsevier B.V.

authors

• Hernández-Arteaga A.
• José-Yacamán M.
• Navarro Contreras Hugo Ricardo
• Ojeda-Galván H.J.
• Rodríguez Aranda Ma. del Carmen
• Sanchez J.E.
• Toro Vázquez Jorge Fernando

publication date

• 2022-01-01

funding provided via

• Laboratorio Nacional de Análisis Físicos, Químicos y Biológicos-UASLP (LANAFQB); Consejo Nacional de Ciencia y Tecnología, CONACYT poroyecto Mantenimiento de Infraestructura Científica en Laboratorios Nacionales 2020-314931, 2021-315911; National Science Foundation, NSF: 2030488  Grant

published in

• Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy  Journal

keywords

• Denaturation temperature; Glycoprotein Spike; S1 subunit; SARS-CoV-2 virus Denaturation; Proteins; Raman spectroscopy; Viruses; % reductions; Denaturation temperatures; Glycoprotein spike; Peak intensity; Raman bands; S1 subunit; SARS-CoV-2 virus; Spectra's; Spike protein; Temperature response; Glycoproteins

Digital Object Identifier (DOI)

• 10.1016/j.saa.2021.120269

PubMed ID

start page

end page

volume

• 264

issue