Morphological and biological properties of silica nanoparticles for CRTC3-siRNA delivery and downregulation of the RGS2 expression in preadipocytes
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The aim of this study was to characterize the morphological properties of amorphous silica nanoparticles (SiO2 NPs), their cytotoxicity and intracellular location within Human Osteoblasts (HOB). Additionally, SiO2 NPs were explored for their effectivity as carriers of CRTC3-siRNA on Human Preadipocytes (HPAd), and thus downregulate RGS2 gene expression. SiO2 NPs were synthesized using the method of Stöber at 45 °C, 56 °C, and 62 °C. These were characterized via TEM with EDS, Zeta Potential and FT-IR. Cytotoxicity was evaluated by XTT at three concentrations 50, 100 and 500 µg/mL; SiO2 NPs intracellular localization was observed through Confocal Laser Scanning Microscope. Delivering siRNA effectivity was measured by RT-qPCR. Morphology of SiO2 NPs was spherical with a range size from 64 to 119 nm; their surface charge was negative. Confocal images demonstrated that SiO2 NPs were located within cellular cytoplasm. At a SiO2 NPs concentration of 500 µg/mL HOB viability decreased, while at 50 µg/mL and 100 µg/mL cell viability was not affected regardless SiO2 NPs size. SiO2 NPs-CRTC3-siRNA are effective to down-regulate RGS2 gene expression in HPAd without cytotoxic effects. The developed SiO2 NPs-CRTC3-siRNA are a promising tool as a delivery vehicle to control obesity. © The Author(s) 2021.
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amorphous silica nanoparticles; CRTC3; Obesity; RGS2; siRNA Cytology; Gene expression; Morphology; Nanoparticles; Polymerase chain reaction; Silica nanoparticles; Silicon; SiO2 nanoparticles; Amorphous silica; Biological properties; Confocal laser scanning microscope; Cytotoxic effects; Delivery vehicle; Human osteoblast; Intracellular localization; Morphological properties; Silica; antineoplastic agent; CRTC3 protein, human; nanoparticle; RGS protein; RGS2 protein, human; silicon dioxide; small interfering RNA; transcription factor; cell survival; chemistry; confocal microscopy; down regulation; drug delivery system; drug effect; gene knockdown; genetics; human; infrared spectroscopy; metabolism; osteoblast; particle size; Antineoplastic Agents; Cell Survival; Down-Regulation; Drug Delivery Systems; Gene Knockdown Techniques; Humans; Microscopy, Confocal; Nanoparticles; Osteoblasts; Particle Size; RGS Proteins; RNA, Small Interfering; Silicon Dioxide; Spectroscopy, Fourier Transform Infrared; Transcription Factors
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