B regulatory cells associated with changes in biochemical and inflammatory parameters in normal-glycemic individuals, pre-diabetes and T2DM patients Article uri icon

abstract

  • Aim: To determine the percentages of (CD19 CD24 CD38 , CD19 CD24 CD27 , CD19 IL-10 )-Breg cells, IL-17 single and IL-17 /IFN-γ double producers T cells and IFN-γ T cells, in normal-glycemic individuals, prediabetes and T2DM patients, and to analyze the association of Breg cells with metabolic parameters of T2DM. Methods: percentages of Breg cells, IL-17 and IL-17 IFN-γ T cells, IFN-γ T cells and IL-10 were determined by flow cytometry. IL-6 levels were evaluated by ELISA assay. Results: increased IL-6 levels, IL-17 and IL-17 IFN-γ T cells and a diminution of IL-10 levels and CD19 IL-10 cells in T2DM patients were observed. We found that CD19 CD24 CD27 cells and CD19 CD24 CD38 cells were increased in T2DM patients. The percentages of CD19 CD24 CD38 cells were associated with HOMA-B, TyG index, HDL and cholesterol values. In normal-glycemic individuals, CD19 CD24 CD27 cells were inversely associated to triglycerides and TyG index. In prediabetes patients, CD19 CD24 CD38 cells were inversely related with cholesterol and LDL. Finally, CD19 CD24 CD38 cells were inversely related with HDL values in T2DM patients. Conclusion: Our results suggest that increased percentages of IL-17 single and IL-17/IFN-γ double producers T cells in T2DM patients may be a consequence of the initial CD19 IL-10 cells reduction. Furthermore, dyslipidemia could play an important role in percentages and activity of B regulatory cells. © 2021 Elsevier B.V.
  • Aim: To determine the percentages of (CD19 %2b CD24 %2b CD38%2b, CD19 %2b CD24 %2b CD27%2b, CD19 %2b IL-10%2b)-Breg cells, IL-17 single and IL-17%2b/IFN-γ double producers T cells and IFN-γ%2b T cells, in normal-glycemic individuals, prediabetes and T2DM patients, and to analyze the association of Breg cells with metabolic parameters of T2DM. Methods: percentages of Breg cells, IL-17%2b and IL-17 %2b IFN-γ%2b T cells, IFN-γ%2b T cells and IL-10 were determined by flow cytometry. IL-6 levels were evaluated by ELISA assay. Results: increased IL-6 levels, IL-17%2b and IL-17 %2b IFN-γ%2b T cells and a diminution of IL-10 levels and CD19 %2b IL-10%2b cells in T2DM patients were observed. We found that CD19 %2b CD24 %2b CD27%2b cells and CD19 %2b CD24 %2b CD38%2b cells were increased in T2DM patients. The percentages of CD19 %2b CD24 %2b CD38%2b cells were associated with HOMA-B, TyG index, HDL and cholesterol values. In normal-glycemic individuals, CD19 %2b CD24 %2b CD27%2b cells were inversely associated to triglycerides and TyG index. In prediabetes patients, CD19 %2b CD24 %2b CD38%2b cells were inversely related with cholesterol and LDL. Finally, CD19 %2b CD24 %2b CD38%2b cells were inversely related with HDL values in T2DM patients. Conclusion: Our results suggest that increased percentages of IL-17 single and IL-17/IFN-γ double producers T cells in T2DM patients may be a consequence of the initial CD19 %2b IL-10%2b cells reduction. Furthermore, dyslipidemia could play an important role in percentages and activity of B regulatory cells. © 2021 Elsevier B.V.

publication date

  • 2021-01-01