Macrophage migration inhibitory factor gene polymorphisms as exacerbating factors of apical periodontitis
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Background. Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5-8 and -173G>C); however, so far there are no reports of studies related to oral health. Objectives. To genotype the -794 CATT5-8 and -173G>C MIF polymorphisms in Mexican patients with apical periodontitis as a genetic risk of exacerbation. Material and methods. The study involved 120 patients with apical periodontitis: 60 with a diagnosis of acute apical periodontitis (Group A) and 60 without previous episodes of exacerbation (Group B). Allelic discrimination was performed from peripheral blood DNA; the repeat polymorphism -794 CATT5-8 was genotyped with sequencing, while the -173G>C polymorphism was determined using real-time polymerase chain reaction (RT-PCR) using TaqMan probes. The associations between MIF polymorphisms, haplotypes and the risk of exacerbated apical periodontitis were assessed. Results. The allele CATT7 was associated with the risk of a stage of acute inflammation (OR = 4.13; 95%25 CI = 1.82-9.63; p =< 0.001). Regarding the -173G >C polymorphism, a process of inflammation exacerbation was only associated with the CC genotype (OR = 4.1; 95%25 CI = 1.02-20.84; p = 0.045). The analysis of the haplotype showed that the combination CATT7/C increases the risk of exacerbation of apical periodontitis (OR = 3.57; 95%25 CI = 1.038-13.300; p = 0.021). Conclusions. The polymorphisms -794 CATT5-8 and -173G>C MIF seem to significantly influence the development of a state of exacerbated inflammation in patients with apical periodontitis. © 2020 Wroclaw University of Medicine. All rights reserved.
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Apical periodontitis; Genetic polymorphisms; Macrophage migration inhibitory factor macrophage migration inhibition factor; macrophage migration inhibition factor; adult; aged; allele; Article; controlled study; cross-sectional study; disease exacerbation; female; gene sequence; genetic polymorphism; genetic risk; genotype; haplotype; human; inflammation; major clinical study; male; Mexican; real time polymerase chain reaction; risk assessment; tooth periapical disease; blood; gene frequency; genetic predisposition; genetics; single nucleotide polymorphism; tooth periapical disease; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; Macrophage Migration-Inhibitory Factors; Periapical Periodontitis; Polymorphism, Genetic; Polymorphism, Single Nucleotide
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