Rapid production in maize seedlings of the Ag85B antigen of Mycobacterium avium subsp. paratuberculosis using an Agrobacterium-mediated transient expression system
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Transient expression systems for plants have several advantages for the development of innovative plant-made vaccines. This technology has the potential to render veterinary vaccines against relevant diseases, such as paratuberculosis, a chronic intestinal infectious disease affecting livestock. This study showed that Agrobacterium-mediated transient transformation of maize seedlings allows the production of the Ag85B antigen from Mycobacterium avium subsp. paratuberculosis (MAP) at levels up to 11.35 µg g−1 dry weight. Moreover, BALB/c mice orally and subcutaneously immunized with the maize-made Ag85B vaccine produced specific IgG and IgA antibodies. Therefore, this platform is proposed as a rapid approach to produce the functional MAPAg85B antigen at sufficient yields to induce specific humoral immune responses in animal testing, which constitutes the first step in the development of a plant-made oral vaccine against paratuberculosis. © 2020, Springer Nature B.V.
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Heterologous protein; Maize; MAPAg85B; Oral vaccines; Transient transformation Agriculture; Antigens; Diseases; Mammals; Vaccines; Agrobacterium mediated; Heterologous proteins; Humoral immune response; Maize; MAPAg85B; Mycobacterium avium subsp. paratuberculosis; Plant-made vaccines; Transient expression; Silver compounds; Agriculture; Corn; Development; Diseases; Mammals; Production; Silver Compounds
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