Levels of Myeloperoxidase and Metalloproteinase-9 in Gingival Crevicular Fluid from Diabetic Subjects with and without Stage 2, Grade B Periodontitis
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Objective. The present study aimed to compare levels of matrix metalloproteinase-9 (MMP-9) and myeloperoxidase (MPO) in gingival crevicular fluid (GCF) from subjects with controlled and noncontrolled Type 2 Diabetes Mellitus (T2D), with and without stage 2 grade B periodontitis (POD2B) versus healthy (H) subjects. Methods. The levels of both enzymes, from 80 GCF samples collected with PerioPaper strips, were analyzed by a Multiplex/Luminex assay. Five groups were formed, all current patients at the Institutional Dentistry Service, and distributed as follows: two groups of diabetics (one controlled and one poorly controlled); two groups with the previous conditions and diagnosed with POD2B; and one H group. Results. The highest concentration of MMP-9 corresponded to the H group, while the lowest corresponded to the T2D controlled group. Regarding MPO levels, the highest levels were associated with the T2D controlled with POD2B group and the lowest with the T2D controlled group. Conclusions. No apparent relationship between the elevation of MMP-9 and MPO levels was observed among subjects with T2D, with and without POD2B, compared to H subjects. © 2019 Diana C. Peniche-Palma et al.
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gelatinase B; hemoglobin A1c; myeloperoxidase; gelatinase B; MMP9 protein, human; MPO protein, human; peroxidase; adult; aged; antibiotic therapy; Article; clinical examination; controlled study; diabetes mellitus; diabetic patient; female; gingivitis; hemoglobin blood level; human; major clinical study; male; non insulin dependent diabetes mellitus; non invasive procedure; periodontitis; clinical trial; enzymology; gingivitis; metabolism; middle aged; non insulin dependent diabetes mellitus; periodontitis; Adult; Aged; Diabetes Mellitus, Type 2; Female; Gingival Crevicular Fluid; Humans; Male; Matrix Metalloproteinase 9; Middle Aged; Periodontitis; Peroxidase
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