abstract

• Introduction. Silver nanoparticles are used in endodontics due to their antimicrobial activity, although it is considered that bacteria are unable to develop resistance to silver nanoparticles. Silver resistance genes have been related to resistance to nanoparticles and antibiotics. The presence of these resistance genes has not been studied in endodontic bacteria. The objective of this study is to report the prevalence of silver resistance genes in endodontic bacteria. Methods. The selected teeth were isolated using a rubber dam and any restoration, post, or caries was eliminated. The operative field was disinfected, and the root-filling material was removed. The samples were obtained using three sterile paper points to absorb the fluid of the root canal. The DNA from the samples and the control organism was extracted, and the detection of the silCBA resistance genes was carried out by PCR. Results. The results of this study show a high prevalence (73.3%25) of silCBA silver resistance genes. The Spearman rank correlation coefficient was utilized to identify correlations between the presence of genes and clinical variables. Conclusions. This study reports a high frequency of silver resistance genes related to nanoparticle resistant from bacteria. © 2019 Marco Felipe Salas-Orozco et al.

authors

• Martínez Castañón Gabriel Alejandro
• Niño Martínez Nereyda
• Patiño Marín Nuria
• Ruiz Facundo
• Salas-Orozco M.F.
• Torres Méndez Fernando

publication date

• 2019-01-01

funding provided via

• 511-6/18-11856, C19-FAI-05-67.67  Grant
• Consejo Nacional de Ciencia y Tecnología, CONACYT: 460066  Grant

published in

• Journal of Nanomaterials  Journal

keywords

• Bacteria; Genes; Metal nanoparticles; Polymerase chain reaction; Anti-microbial activity; Filling materials; High frequency HF; Resistance genes; Root canals; Rubber dams; Silver resistance; Spearman rank correlation; Silver nanoparticles

Digital Object Identifier (DOI)

• 10.1155/2019/8742975

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volume

• 2019