abstract

• Scaffolds have been used as extracellular matrix analogs to promote cell migration, cell attachment, and cell proliferation. The use of aerogels and carbon-based nanomaterials has recently been proposed for tissue engineering due to their properties. The aim of this study is to develop a highly porous collagen-alginate(-graphene oxide) aerogel-based scaffold. The GO synthesis was performed by Hummers method; a collagen-alginate and collagen-alginate-GO hydrogel were synthetized; then, they were treated by a supercritical drying process. The aerogels obtained were evaluated by SEM and FTIR. Osteoblasts were seeded over the scaffolds and evaluated by SEM. According to the characterization, the aerogels showed a highly porous interconnected network covered by a nonporous external wall. According to the FTIR, the chemical functional groups of collagen and GO were maintained after the supercritical process. The SEM images after cell culture showed that a collagen-alginate scaffold promotes cell attachment and proliferation. The alginate-collagen aerogel-based scaffold could be a platform for tissue engineering since it shows adequate properties. Further studies are needed to determine the cell interactions with GO. © 2019 Abraham Muñoz-Ruíz et al.

authors

• Escobar-García D.M.
• Flores Reyes Héctor Eduardo
• Muñoz-Ruíz A.
• Pozos Guillén Amaury de Jesús
• Quintana Ruiz Mildred

publication date

• 2019-01-01

funding provided via

• Consejo Nacional de Ciencia y Tecnología, CONACYT: 289207  Grant

published in

• Journal of Nanomaterials  Journal

keywords

• Aerogels; Alginate; Cell culture; Cell proliferation; Cells; Collagen; Graphene; Tissue; Alginate aerogels; Alginate scaffolds; Chemical functional groups; Extracellular matrices; Interconnected network; Supercritical drying; Supercritical process; Synthesis and characterizations; Scaffolds (biology)

Digital Object Identifier (DOI)

• 10.1155/2019/2875375

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volume

• 2019