abstract

• Introduction: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. Methods: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. Results: The rotating method was easier, faster, and more sensitive than the others with 100%25 specificity. Conclusions: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities. © 2019, Sociedade Brasileira de Medicina Tropical. All rights reserved.

authors

• Carlier Y.
• Gamboa León Miriam Rubí de los Milagros
• Meza-González G.
• Truyens C.
• Vera-Ku M.

publication date

• 2019-01-01

published in

• Revista da Sociedade Brasileira de Medicina Tropical  Journal

keywords

• Capillary tube test; Microhematocrit test; T. Cruzi diagnostic methods; Trypanosoma cruzi Article; blood sampling; Chagas disease; comparative study; congenital Chagas disease; diagnostic procedure; health care facility; hematocrit; laboratory personnel; methodology; microscopy; nonhuman; parasite; parasitemia; rotating method; rotation; sensitivity and specificity; Trypanosoma cruzi; World Health Organization; animal; blood; capillary tube; centrifugation; Chagas disease; clinical laboratory service; comparative study; hematocrit; human; isolation and purification; parasitemia; parasitology; procedures; Trypanosoma cruzi; Animals; Capillary Tubing; Centrifugation; Chagas Disease; Clinical Laboratory Services; Hematocrit; Humans; Parasitemia; Sensitivity and Specificity; Trypanosoma cruzi

Digital Object Identifier (DOI)

• 10.1590/0037-8682-0505-2018

PubMed ID

• 31271620

start page

end page

volume

• 52