Synthesis of amino acid-naphthoquinones and in vitro studies on cervical and breast cell lines
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We performed an extensive analysis about the reaction conditions of the 1,4-Michael addition of amino acids to 1,4-naphthoquinone and substitution to 2,3-dichloronaphthoquinone, and a complete evaluation of stoichiometry, use of different bases, and the pH influence was performed. We were able to show that microwave-assisted synthesis is the best method for the synthesis of naphthoquinone-amino acid and chloride-naphthoquinone-amino acid derivatives with 79-91%25 and 78-91%25 yields, respectively. The cyclic voltammetry profiles showed that both series of naphthoquinone-amino acid derivatives mainly display one quasi-reversible redox reaction process. Interestingly, it was shown that naphthoquinone derivatives possess a selective antitumorigenic activity against cervix cancer cell lines and chloride-naphthoquinone-amino acid derivatives against breast cancer cell lines. Furthermore, the newly synthetized compounds with asparagine-naphthoquinones (3e and 4e) inhibited ∼85%25 of SiHa cell proliferation. These results show promising compounds for specific cervical and breast cancer treatment. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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Alternative methods; Amino acids; Anticancer; Microwave; Naphthoquinone; Ultrasound amino acid; antineoplastic agent; naphthoquinone; breast tumor; cell proliferation; chemistry; drug effect; drug screening; female; human; synthesis; tumor cell line; uterine cervix tumor; Amino Acids; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Drug Screening Assays, Antitumor; Female; Humans; Naphthoquinones; Uterine Cervical Neoplasms
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