Antimicrobial effect of a hyperosmotic solution on endodontic microorganisms in planktonic state. [Efecto antimicrobiano de una solución hiperosmótica sobre microorganismos endodónticos en estado planctónico.] Article uri icon

abstract

  • To achieve the elimination of microorganisms inside root canals with endodontic treatment, the use of a suitable mechanical and chemical bio-preparation is necessary. The chemical irrigation is performed with endodontic irrigation solutions, the most commonly used is sodium hypochlorite; however, this has harmful effects as cytotoxicity and thus, the research of new solutions is essential in this area. The aim of this study was to evaluate the anti-microbial effect ex-vivo of a hyperosmotic solution based on salts of potassium and sodium. A mixture of endodontic microorganisms (Enterococcus faecalis and Candida albicans) was employed at different concentrations and with 5, 10 and 15 minutes of contact time with the hyperosmotic solution and subsequently, counts of colony forming units were carried out. In addition, the percent of microbial inhibition of the hyperosmotic solution was evaluated in comparison to 5.25%25 sodium hypochlorite. A significant difference was found in the amount of bacterial load after the use of the hyperosmotic solution, and the percent of microbial reduction of this solution was similar to 5.25%25 sodium hypochlorite. This study showed that the hyperosmotic solution has a potent anti-microbial effect against endodontic microorganisms in planktonic state, thus it could be used as endodontic irrigation agent, following further ex-vivo investigations. © 2019, Instituto de Investigaciones Clinicas. All rights reserved.

publication date

  • 2019-01-01