Frequency of regulatory B cells in adipose tissue and peripheral blood from individuals with overweight, obesity and normal-weight Article uri icon

abstract

  • Statements of the problem: Regulatory B (Breg) cells have a critical role in adipose tissue homeostasis, and although subtypes of Breg cells have been described, their contribution during obesity is unclear. Therefore, the levels of regulatory B cells in adipose tissue and peripheral blood samples drawn from individuals with overweight, obesity, and normal-weight were evaluated. Methods: The percentages of Breg cells were analyzed by flow cytometry. The activity of Breg cells was assessed by measuring the release of IFN-γ in the supernatants of co-cultures of CD4 T and regulatory B cells with an ELISA assay. The levels of IL-17 and IFN-γ produced by the CD4 T cells were assessed using an ELISA assay. Results: Diminished frequencies of Breg cells with phenotypes CD19 CD27 CD38High, CD19 CD24HighCD38High, and CD19 CD24HighCD38HighIL-10 cells were observed in the blood samples from the individuals with overweight and obesity but not in the individuals with normal-weight. The production of IFN-γ in CD4 T-cell cultures showed a decrease in the presence of Breg cells in individuals with obesity and normal-weight. We found fewer percentages of CD19 CD27 CD38High cells in the adipose tissue samples from individuals with overweight and obesity compared to individuals with normal-weight. In addition, elevated levels of IL-17 and IFN-γ in the supernatants of the cultures of CD4 T cells from the individuals with obesity compared to the individuals with normal-weight were observed. Conclusions: The results suggest that individuals with obesity show increased levels of Th1/Th17 cytokines, which might be caused by the decreased frequency of regulatory B cells. © 2018 Asia Oceania Association for the Study of Obesity
  • Statements of the problem: Regulatory B (Breg) cells have a critical role in adipose tissue homeostasis, and although subtypes of Breg cells have been described, their contribution during obesity is unclear. Therefore, the levels of regulatory B cells in adipose tissue and peripheral blood samples drawn from individuals with overweight, obesity, and normal-weight were evaluated. Methods: The percentages of Breg cells were analyzed by flow cytometry. The activity of Breg cells was assessed by measuring the release of IFN-γ in the supernatants of co-cultures of CD4%2b T and regulatory B cells with an ELISA assay. The levels of IL-17 and IFN-γ produced by the CD4%2b T cells were assessed using an ELISA assay. Results: Diminished frequencies of Breg cells with phenotypes CD19%2bCD27%2bCD38High, CD19%2bCD24HighCD38High, and CD19%2bCD24HighCD38HighIL-10%2b cells were observed in the blood samples from the individuals with overweight and obesity but not in the individuals with normal-weight. The production of IFN-γ in CD4%2b T-cell cultures showed a decrease in the presence of Breg cells in individuals with obesity and normal-weight. We found fewer percentages of CD19%2bCD27%2bCD38High cells in the adipose tissue samples from individuals with overweight and obesity compared to individuals with normal-weight. In addition, elevated levels of IL-17 and IFN-γ in the supernatants of the cultures of CD4%2b T cells from the individuals with obesity compared to the individuals with normal-weight were observed. Conclusions: The results suggest that individuals with obesity show increased levels of Th1/Th17 cytokines, which might be caused by the decreased frequency of regulatory B cells. © 2018 Asia Oceania Association for the Study of Obesity

publication date

  • 2018-01-01