Characterization of biaxial stretch as an in vitro model of traumatic brain injury to the blood-brain barrier
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Traumatic brain injury (TBI) is one of the major causes of disability in the USA. It occurs when external mechanical forces induce brain damage that causes deformation of brain tissue. TBI is also associated with alterations of the blood-brain barrier (BBB). Using primary rat brain microvascular endothelial cells as an in vitro BBB model, the effects of biaxial stretch were characterized at 5, 10, 15, 25, and 50%25 deformation using a commercially available system. The results were compared to the effects of mild and moderate TBI in vivo, induced by the weight-drop method in mice. In vitro, live/dead cells, lactate dehydrogenase (LDH) release, caspase 3/7 staining, and tight junction (TJ) protein expression were evaluated 24 h after a single stretch episode. In vivo, Evans blue extravasation, serum levels of S100β, and TJ protein expression were evaluated. Stretch induced a deformationdependent increase in LDH release, cell death, and activation of caspase 3/7, suggesting the induction of apoptosis. Interestingly, low magnitudes of deformation increased the expression of TJ proteins, likely in an attempt to compensate for stretch damage. High magnitudes of deformation decreased the expression of TJ proteins, suggesting that the damage was too severe to counteract. In vivo, mild TBI did not affect BBB permeability or the expression of TJ proteins. However, moderate TBI significantly increased BBB permeability and decreased the expression of these proteins, similar to the results obtained with a high magnitude deformation. These data support the use biaxial stretch as valuable tool in the study of TBI in vitro. © US Government (outside the USA) 2017.
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Biaxial stretch; Blood-brain barrier; In vitro models; Traumatic brain injury caspase 3; caspase 7; lactate dehydrogenase; protein S100B; tight junction protein; animal cell; animal experiment; apoptosis; Article; biaxial stretch; biomechanics; blood brain barrier; capillary endothelial cell; cell death; comparative study; controlled study; disease severity; enzyme release; extravasation; head injury; in vitro study; in vivo study; necrosis; nonhuman; protein blood level; protein expression; rat; traumatic brain injury; animal; blood brain barrier; capillary permeability; disease model; metabolism; pathology; physiology; Sprague Dawley rat; tight junction; traumatic brain injury; vascular endothelium; Animals; Blood-Brain Barrier; Brain Injuries, Traumatic; Capillary Permeability; Disease Models, Animal; Endothelium, Vascular; Rats; Rats, Sprague-Dawley; Tight Junctions
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