abstract

• The new era of biomaterials for repairing bone tissue injury continues to be a challenge in bone tissue engineering. The fiber scaffolds allow for cellular interconnection and a microenvironment close to the bone extracellular matrix. The aim of this study was to evaluate the osteoblast behavior on a 3D textile of PGA (polyglycolic acid) fibers functionalized with the RGD (R: arginine; G: glycine; D: aspartic acid) peptide. The cell morphology, proliferation, and calcium phosphate deposition ability were evaluated on textiles at different time intervals under a confocal laser scanning microscope. The osteoblast viability ranged from 92%25 to 98%25, and cell proliferation was higher in PGA-RGD than control PGA (uncoated). In addition, the osteoblast calcium phosphate deposition was significantly greater on PGA-RGD in osteogenic inductor medium (OIM) in contrast to controls without inducing factors. The PGA-RGD fibers supported proliferation and viability of osteoblast and stimulated bone osteogenesis and mineralization. These results support the adoption of this 3D polymeric textile as a scaffold for bone tissue engineering. © 2017 Mariné Ortiz et al.

authors

• Escobar-García D.M.
• Flores Reyes Héctor Eduardo
• Grandfils C.
• Ortiz M.
• Pozos Guillén Amaury de Jesús
• Álvarez-Pérez M.A.

publication date

• 2017-01-01

published in

• Journal of Nanomaterials  Journal

keywords

• Amino acids; Bone; Calcium; Calcium compounds; Calcium phosphate; Cell proliferation; Deposition; Enzyme activity; Textiles; Tissue; Tissue engineering; Bone regeneration; Bone tissue engineering; Calcium phosphate deposition; Confocal laser scanning microscope; Extracellular matrices; Inducing factors; Microenvironments; Poly glycolic acid; Scaffolds (biology)

Digital Object Identifier (DOI)

• 10.1155/2017/4852190

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volume

• 2017