The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse Article uri icon

abstract

  • The use of corn smut for the production of recombinant vaccines has been recently implemented by our group. In this study, the stability and immunogenic properties of the corn smut-based cholera vaccine, based on the cholera toxin B subunit (CTB), were determined in mouse. The immunogenic potential of distinct corn smut CTB doses ranging from 1 to 30 μg were assessed, with maximum humoral responses at both the systemic (IgG) and intestinal (IgA) levels at a dose of 15 μg. The humoral response last for up to 70 days after the third boost. Mice were fully protected against a challenge with cholera toxin after receiving three 15 μg-doses. Remarkably, the corn smut-made vaccine retained its immunogenic activity after storage at room temperature for a period of 1 year and no reduction on CTB was observed following exposure at 50 °C for 2 h. These data support the use of the corn smut-made CTB vaccine as a highly stable and effective immunogen and justify its evaluation in target animal models, such as piglet and sheep, as well as clinical evaluations in humans. © 2016 Elsevier B.V.

publication date

  • 2016-01-01