abstract

• The objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydroxyapatite was doped with 3, 5, 10, and 20 wt%25 of europium. The obtained samples were characterized after they were dried at 80°C and hydrothermal treated at 120°C by 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and photoluminescence. Also, impregnation and release of 5-fluorouracil were assessed in PBS. The toxicity effects of all samples were studied using viability assays on human fibroblasts cells (HGF-1) in vitro. The sizes of the crystallites were about 10-70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9-0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5%25 of europium and loaded with 5-fluorouracil release almost 7 mg/L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours. © 2016 Paulina-Guadalupe Miranda-Meléndez et al.

authors

• Casillas-Santana M.-A.
• Castillo-Silva B.-E.
• Martínez Castañón Gabriel Alejandro
• Miranda-Meléndez P.-G.
• Niño Martínez Nereyda
• Patiño Marín Nuria
• Ruiz Facundo

publication date

• 2016-01-01

published in

• Bioinorganic Chemistry and Applications  Journal

Digital Object Identifier (DOI)

• 10.1155/2016/1057260

start page

end page

volume

• 2016