Simulated shift work in rats perturbs multiscale regulation of locomotor activity
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Motor activity possesses a multiscale regulation that is characterized by fractal activity fluctuations with similar structure across a wide range of timescales spanning minutes to hours. Fractal activity patterns are disturbed in animals after ablating the master circadian pacemaker (suprachiasmatic nucleus, SCN) and in humans with SCN dysfunction as occurs with aging and in dementia, suggesting the crucial role of the circadian system in the multiscale activity regulation.We hypothesized that the normal synchronization between behavioural cycles and the SCN-generated circadian rhythms is required for multiscale activity regulation. To test the hypothesis,we studied activity fluctuations of rats in a simulated shift work protocol that was designed to force animals to be active during the habitual resting phase of the circadian/daily cycle. We found that these animals had gradually decreased mean activity level and reduced 24-h activity rhythm amplitude, indicating disturbed circadian and behavioural cycles. Moreover, these animals had disrupted fractal activity patterns as characterized by more random activity fluctuations at multiple timescales from 4 to 12 h. Intriguingly, these activity disturbances exacerbated when the shift work schedule lasted longer and persisted even in the normal days (without forced activity) following the shift work. The disrupted circadian and fractal patterns resemble those of SCN-lesioned animals and of human patients with dementia, suggesting a detrimental impact of shift work on multiscale activity regulation. © 2014 The Author(s) Published by the Royal Society. All rights reserved.
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Circadian misalignment; Multiscale regulation; Shift work Fractals; Neurodegenerative diseases; Rats; Activity disturbances; Activity regulation; Circadian misalignment; Locomotor activity; Multiple timescales; Multiscale regulation; Shift work; Suprachiasmatic nucleus; Animals; animal experiment; article; circadian rhythm; controlled study; fractal analysis; hypothalamus function disorder; light dark cycle; locomotion; nonhuman; rat; shift worker; simulation; suprachiasmatic nucleus; work schedule; animal; circadian rhythm; motor activity; photoperiodicity; physiology; Wistar rat; Animals; Circadian Rhythm; Motor Activity; Photoperiod; Rats; Rats, Wistar; Suprachiasmatic Nucleus; Work Schedule Tolerance
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