Adherence inhibition of Streptococcus mutans on dental enamel surface using silver nanoparticles
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The aim of this ex vivo study was to evaluate the adherence capacity of Streptococcus mutans after being exposed to three different sizes of silver nanoparticles on healthy human dental enamel. Three different sizes of silver nanoparticles (9.3, 21.3 and 98 nm) were prepared, characterized and an adherence testing was performed to evaluate their anti-adherence activity on a reference strain of S. mutans on healthy dental enamel surfaces. Colony-Forming Unit count was made for adherence test and light microscopy, atomic force microscopy and scanning electron microscopy were used to compare qualitative characteristics of S. mutans. 9.3 nm and 21.3 nm groups did not show differences between them but statistical differences were found when 9.3 nm and 21.3 nm groups were compared with 98 nm and negative control groups (p < 0.05). Microscopy analysis shows a better inhibition of S. mutans adherence in 9.3 nm and 21.3 nm groups than the 98 nm group when compared with control group. Silver nanoparticles showed an adherence inhibition on S. mutans and the anti-adherence capacity was better when silver nanoparticles were smaller. © 2013 Elsevier B.V.
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Adherence inhibition; Antibacterial effect; Dental enamel; Inhibitory effect; Silver nanoparticles; Streptococcus mutans Antibacterial effects; Dental enamels; Inhibitory effect; Silver nanoparticles; Streptococcus mutans; Atomic force microscopy; Enamels; Nanoparticles; Scanning electron microscopy; Silver; metal nanoparticle; silver; metal nanoparticle; silver; article; atomic force microscopy; bacterium adherence; chemistry; cytology; drug effect; enamel; human; light; microbial sensitivity test; radiation scattering; Streptococcus mutans; surface property; ultrastructure; bacterium adherence; chemistry; cytology; drug effects; enamel; Streptococcus mutans; Bacterial Adhesion; Dental Enamel; Humans; Light; Metal Nanoparticles; Microbial Sensitivity Tests; Microscopy, Atomic Force; Scattering, Radiation; Silver; Streptococcus mutans; Surface Properties; Bacterial Adhesion; Dental Enamel; Humans; Light; Metal Nanoparticles; Microbial Sensitivity Tests; Microscopy, Atomic Force; Scattering, Radiation; Silver; Streptococcus mutans; Surface Properties
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