Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation Article uri icon

abstract

  • Pyrostegia venusta (Ker Gawl.) Miers is used in traditional medicine for the treatment of vitiligo, dysentery, immoderate menstrual flow, common diseases of the respiratory system, and for the treatment of genital infections. This work describes the effect of different explants, media components, and antioxidants on callus development from P. venusta. The effect of osmotic and water stress on flavonoids and sterols content was also evaluated. Best callus formation was obtained when leaves were used as explants and employing Murashige and Skoog medium with 2,4-dichlorophenoxiacetic acid (1 mg l-1), benzyladenine (0.1 mg l-1), and 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (1 mg l-1). Addition of cooper increase development of callus and the mixture of ascorbic acid, citric acid, and polyvinyl pyrrolidone reduced oxidation significantly. Water stress increased 4.6 folds the content of sterols, while osmotic stress induced 3.8 and 2.2 folds sterols and flavonoids concentration, respectively, in relation to leaves. The results suggest that P. venusta callus could be a novel source of bio-compounds for pharmaceutical preparations. © 2012 Society for Plant Biochemistry and Biotechnology.

publication date

  • 2013-01-01