Relationship between serum levels of triglycerides and vascular inflammation, measured as COX-2, in arteries from diabetic patients: A translational study
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Background: Inflammation is a common feature in the majority of cardiovascular disease, including Diabetes Mellitus (DM). Levels of pro-inflammatory markers have been found in increasing levels in serum from diabetic patients (DP). Moreover, levels of Cyclooxygenase-2 (COX-2) are increased in coronary arteries from DP. Methods. Through a cross-sectional design, patients who underwent CABG were recruited. Vascular smooth muscle cells (VSMC) were cultured and COX-2 was measured by western blot. Biochemical and clinical data were collected from the medical record and by blood testing. COX-2 expression was analyzed in internal mammary artery cross-sections by confocal microscopy. Eventually, PGI2 and PGE2 were assessed from VSMC conditioned media by ELISA. Results: Only a high glucose concentration, but a physiological concentration of triglycerides exposure of cultured human VSMC derived from non-diabetic patients increased COX-2 expression.Diabetic patients showed increasing serum levels of glucose, Hb1ac and triglycerides. The bivariate analysis of the variables showed that triglycerides was positively correlated with the expression of COX-2 in internal mammary arteries from patients (r2 = 0.214, P < 0.04). Conclusions: We conclude that is not the glucose blood levels but the triglicerydes leves what increases the expression of COX-2 in arteries from DP. © 2013 Gordillo-Moscoso et al.; licensee BioMed Central Ltd.
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COX-2; Diabetes mellitus; Human arteries; Lipid profile apolipoprotein A; C reactive protein; cholesterol; creatinine; cyclooxygenase 2; fibrinogen; gelatinase B; glucose; hemoglobin A1c; high density lipoprotein cholesterol; homocysteine; interleukin 6; low density lipoprotein cholesterol; prostacyclin; prostaglandin E2; triacylglycerol; tumor necrosis factor alpha; adult; aged; article; cholesterol blood level; confocal microscopy; controlled study; coronary artery; creatinine blood level; cross-sectional study; diabetic patient; enzyme linked immunosorbent assay; female; fibrinogen blood level; glucose blood level; human; human cell; inflammation; major clinical study; male; non insulin dependent diabetes mellitus; protein expression; smooth muscle fiber; triacylglycerol blood level; vascular smooth muscle cell; Western blotting; Adult; Aged; Aged, 80 and over; Blood Glucose; Coronary Vessels; Cyclooxygenase 2; Diabetes Mellitus; Female; Gene Expression Regulation; Humans; Inflammation; Male; Middle Aged; Triglycerides; Tunica Media
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