abstract

• Background: Low temperatures play key roles in the development of most plant diseases, mainly because of their influence on the expression of various virulence factors in phytopathogenic bacteria. Thus far, studies regarding this environmental parameter have focused on specific themes and little is known about phytopathogenic bacteria physiology under these conditions. To obtain a global view regarding phytopathogenic bacteria strategies in response to physiologically relevant temperature changes, we used DNA microarray technology to compare the gene expression profile of the model bacterial pathogen P. syringae pv. phaseolicola NPS3121 grown at 18°C and 28°C. Results: A total of 236 differentially regulated genes were identified, of which 133 were up-regulated and 103 were down-regulated at 18°C compared to 28°C. The majority of these genes are involved in pathogenicity and virulence processes. In general, the results of this study suggest that the expression profile obtained may be related to the fact that low temperatures induce oxidative stress in bacterial cells, which in turn influences the expression of iron metabolism genes. The expression also appears to be correlated with the profile expression obtained in genes related to motility, biofilm production, and the type III secretion system. Conclusions: From the data obtained in this study, we can begin to understand the strategies used by this phytopathogen during low temperature growth, which can occur in host interactions and disease development. © 2013 Arvizu-Gómez et al.; licensee BioMed Central Ltd.

authors

• Aguilar J.R.P.
• Arvizu-Gómez J.L.
• Hernández Morales Alejandro
• Álvarez-Morales A.

publication date

• 2013-01-01

published in

• BMC Microbiology  Journal

keywords

• Low temperature; P. syringae pv. phaseolicola; Physiology; Phytopathogen; Transcriptional profile article; bacterial cell; bacterial gene; bacterial growth; bacterial strain; bacterial virulence; biofilm; cell motility; DNA microarray; down regulation; gene expression; gene expression profiling; iron metabolism; low temperature; nonhuman; nucleotide sequence; oxidative stress; phaseolicola; Pseudomonas syringae; type III secretion system; upregulation; Cold Temperature; Microarray Analysis; Pseudomonas syringae; Stress, Physiological; Transcriptome

Digital Object Identifier (DOI)

• 10.1186/1471-2180-13-81

PubMed ID

• 23587016

start page

end page

volume

• 13

issue

• 1